In the present study silver nanoparticles fabricated by using leaf extract of Alpinia calcarata. We have also studied the effect of various experimental parameters viz., metal ion concentration, pH and incubation period on nanoparticle biosynthesis. Results of optimization showed that metal ion concentration of 1.5 mM, alkaline pH and incubation period of 12 h were the optimum conditions for metal nanoparticle biosynthesis. Synthesized silver nanoparticles were characterized by UV–Visible spectroscopy, Dynamic light scattering (DLS), Zeta potential analysis, Fourier transform infrared spectroscopy (FTIR), Inductively coupled plasma-optical emission spectrometry (ICP-OES), Transmission electron microscopy (TEM) and X-ray diffraction analysis (XRD). The UV–visible spectrum shows a sharp peak at 420 nm which was due to the surface plasmon resonance of the silver nanoparticles. Effect of several phytochemicals present in A. calcarata, on synthesis of silver nanoparticles was studied by Fourier transform infrared spectroscopy. The results indicate that the flavonoids, phytosterol, quinones and phenolic compounds present in the plant extract plays a major role in formation of silver nanoparticles in their respective ions in solution. Results of TEM and XRD analysis showed that synthesized silver nanoparticles were mostly spherical in shape with an average diameter of 27.2 ± 0.2.5 nm and highly crystalline in nature. Moreover the synthesized silver nanoparticles were also evaluated for their potential antibacterial and antioxidant activities. It showed good antibacterial activity as well as antioxidant activity. Thus the obtained result provides a scientific support that leaf extract of A. calcarata can be used efficiently in the production of potential bioactive silver nanoparticles with several pharmaceutical applications.

中文翻译：

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Alpinia calcarata：制造生物活性银纳米颗粒的潜在来源。

在本研究中，银粉是通过使用Alpinia calcarata的叶提取物制成的。我们还研究了各种实验参数，即金属离子浓度，pH和孵育时间对纳米颗粒生物合成的影响。优化结果表明：1.5 mM的金属离子浓度，碱性pH值和12 h的孵育时间是金属纳米颗粒生物合成的最佳条件。紫外可见光谱，动态光散射（DLS），Zeta电位分析，傅里叶变换红外光谱（FTIR），电感耦合等离子体发射光谱（ICP-OES），透射电子显微镜（TEM）和X射线衍射分析（XRD）。紫外可见光谱在420 nm处显示一个尖峰，这是由于银纳米颗粒的表面等离子体共振引起的。通过傅里叶变换红外光谱法研究了cal菜中存在的几种植物化学物质对银纳米颗粒合成的影响。结果表明，植物提取物中存在的类黄酮，植物甾醇，醌和酚类化合物在溶液中各自离子中的银纳米颗粒形成中起主要作用。TEM和XRD分析的结果表明，合成的银纳米颗粒大部分为球形，平均直径为27.2±0.2.5nm，并且具有高度结晶性。此外，还评估了合成的银纳米颗粒的潜在抗菌和抗氧化活性。它显示出良好的抗菌活性和抗氧化活性。因此，所获得的结果提供了科学的支持，即可以将卡拉卡尔塔的叶提取物有效地用于潜在的具有生物活性的银纳米颗粒的生产中，并具有多种药物应用。