Background

Identifying typical odor-causing compounds is essential for odor problem control in drinking water. In this study, aiming at a major water source reservoir in hot and humid areas in southern China, which encountered seasonable odor problems in recent years, an integrated approach including comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC–TOFMS), flavor profile analysis (FPA) and quantitative real-time polymerase chain reaction (qPCR) was adopted to investigate the odor occurrence.

Results

The results indicated that earthy–musty odor is blamed to the seasonable odor problems, and it is consistent with the complaints results from consumers. Fifty-four typical odor compounds were investigated in the reservoir and twelve were detected, of which, 2-methylisoborneol (2-MIB) was significantly increased during the odor event. Pseudanabaena sp. is the dominant species in the reservoir, which can be further represented by the number of mic gene with qPCR method (R² = 0.746, P < 0.001). Oxygen consumption (COD_Mn) and dissolved organic carbon (DOC) have great influence on growth of Pseudanabaena sp., and the release of 2-MIB from the Pseudanabaena sp. cells is affected by temperature and light.

Conclusion

Our findings demonstrated that 2-MIB is the odor-caused substance in the reservoir and Pseudanabaena sp. is the main 2-MIB producer, which was confirmed as a benthic filamentous algae. Due to COD_Mn and DOC have great influence on Pseudanabaena sp. growth, further measures to reduce the CODMn and DOC input should be performed. We also demonstrated that the 2-MIB release is affected by temperature and light. The risk of sudden increase of 2-MIB will be reduced by raising the depth of water in the reservoir. Our study will improve the understanding of T&O problems in this city, as well as in other hot and humid area.

中文翻译：

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南方湿热地区一处水库源水异味的发生、诊断及可能的缓解措施[J].

背景

识别典型的引起异味的化合物对于控制饮用水中的异味问题至关重要。本研究针对我国南方湿热地区某主要水源水库近年来遇到的季节性臭气问题，采用综合二维气相色谱与飞行时间质谱（GC× GC-TOFMS）、风味特征分析（FPA）和定量实时聚合酶链反应（qPCR）被用来研究气味的发生。

结果

结果表明，泥土霉味归咎于季节性气味问题，这与消费者的投诉结果一致。在水库中调查了 54 种典型气味化合物，检测到 12 种，其中 2-甲基异冰片 (2-MIB) 在气味事件期间显着增加。假单胞菌属 是水库中的优势种，可以进一步用qPCR方法的mic基因数来表示（R ²  = 0.746，P  <0.001）。耗氧量 (COD _{Mn ) 和溶解有机碳 (DOC) 对}假单胞菌的生长和假单胞菌释放 2-MIB有很大影响sp。细胞受温度和光照的影响。

结论

我们的研究结果表明，2-MIB 是水库和Pseudanabaena sp. 中引起气味的物质。是主要的 2-MIB 生产者，被确认为底栖丝状藻类。由于 COD _{Mn和 DOC 对}Pseudanabaena sp.有很大的影响。生长，应采取进一步措施减少 CODMn 和 DOC 输入。我们还证明了 2-MIB 的释放受温度和光照的影响。增加水库水深可以降低2-MIB突然增加的风险。我们的研究将提高对该城市以及其他湿热地区的 T&O 问题的理解。