Introduction: Stored red blood cells (RBCs) undergo storage lesions involving morphological, physiological and biochemical changes. MicroRNAs (miRNAs) have important functions in cell apoptosis and life processes. Therefore, the aim of this study was to explore potential roles of miRNAs in the damage of stored RBCs. Methods: Blood samples were collected from 13 healthy male O-type donors, and leuko-reduced RBCs were divided into fresh RBC group and 20-day storage RBC group. Results: Eight predicted miRNAs with modified expressions with an intersection ≥ 3 were found dysregulated in the 20-day storage RBC group and involved in apoptosis and senescence signaling pathway: miR-31-5p, miR-196a-5p, miR-203a, miR-654-3p and miR-769-3p were increased, while miR-96-5P, miR-150-5P and miR-197-3p were decreased. Evidence associating miR-31-5p, miR-203a, miR-654 and miR-769 to RBCs or blood in general are not available. Conclusions: Dysregulated miRNAs might represent potential biomarkers to identify storage lesions, and their detection might help to evaluate the quality of stored RBCs.

中文翻译：

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与红细胞储存相关的 MicroRNA 失调

简介： 储存的红细胞 (RBC) 会经历涉及形态、生理和生化变化的储存损伤。MicroRNAs (miRNAs) 在细胞凋亡和生命过程中具有重要作用。因此，本研究的目的是探索 miRNA 在储存的红细胞损伤中的潜在作用。方法：采集13名健康男性O型供体血样，将去白细胞红细胞分为新鲜红细胞组和20天贮藏红细胞组。结果：在 20 天储存 RBC 组中发现 8 个具有交叉 ≥ 3 的修饰表达的预测 miRNA 失调并参与细胞凋亡和衰老信号通路：miR-31-5p、miR-196a-5p、miR-203a、miR -654-3p和miR-769-3p增加，而miR-96-5P、miR-150-5P和miR-197-3p减少。与 miR-31-5p、miR-203a、miR-654 和 miR-769 通常无法用于红细胞或血液。结论：失调的 miRNA 可能代表潜在的生物标志物来识别储存损伤，它们的检测可能有助于评估储存的红细胞的质量。