Aquatic organisms respond to environmental challenges such as thermal stress with the rapid induction of highly conserved polypeptides known as stress proteins or heat shock proteins (Hsps). Solar ultraviolet radiation (UVR, 280-400 nm) is an important environmental stressor in marine ecosystems. Here, we present results of experiments conducted with the marine copepod Acartia tonsa to follow the de novo protein synthesis and measure the level of constitutive and inducible isoforms of the Hsp70 gene family of stress proteins after UV exposure. Animals were collected from Tampa Bay, Florida (USA), and exposed to solar radiation (full spectrum), UV-A (320-400 nm) and PAR (400-700 nm), or PAR only, for periods of 0.5-4 h. Controls were kept in the dark. Protein synthesis was robust under all treatments when the copepods were exposed to low solar radiation intensities. Conversely, high solar radiation intensities (both UV-B and UV-A) caused an overall suppression in the protein synthesis of the copepods with no detectable induction of stress-inducible isoforms of Hsps. Immunochemical assays (western blotting) showed that UVR increased levels (3.5-4-fold increase compared to the dark control) of the constitutively expressed 70 kDa heat-shock (Hsc70) protein in A. tonsa, without indication of inducible isoform upregulation.

中文翻译：

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亚致死压力：太阳紫外线辐射对桡足类 Acariatonsa 蛋白质合成的影响

水生生物通过快速诱导被称为应激蛋白或热休克蛋白 (Hsps) 的高度保守多肽来应对环境挑战，例如热应激。太阳紫外线辐射（UVR，280-400 nm）是海洋生态系统中重要的环境压力源。在这里，我们展示了对海洋桡足类动物 Acariatonsa 进行的实验结果，以跟踪从头蛋白质合成并测量紫外线照射后 Hsp70 基因家族应激蛋白的组成型和诱导型同种型的水平。从佛罗里达州坦帕湾（美国）收集动物，并暴露于太阳辐射（全光谱）、UV-A（320-400 nm）和 PAR（400-700 nm）或仅 PAR，时间为 0.5-4 H。控制被保持在黑暗中。当桡足类暴露在低太阳辐射强度下时，蛋白质合成在所有处理下都很稳定。相反，高太阳辐射强度（UV-B 和 UV-A）导致桡足类蛋白质合成的整体抑制，没有可检测到的 Hsps 应激诱导同种型的诱导。免疫化学测定（蛋白质印迹）显示 UVR 增加了 A.tonsa 中组成性表达的 70 kDa 热休克 (Hsc70) 蛋白的水平（与黑暗对照相比增加了 3.5-4 倍），没有可诱导的同种型上调的迹象。