Raster-scan optoacoustic angiography at 532 nm wavelength with 50 μm lateral resolution at 2 mm diagnostic depth was used for quantitative characterization of neoangiogenesis in colon cancer models. Two tumor models of human colon adenocarcinoma (HT-29) and murine colon carcinoma (CT26) different in their histology and vascularization were compared. Tumors of both origins showed an inhomogeneous distribution of areas with high and low vascularization. Rapidly growing CT26 tumor demonstrated a higher rate of vessel growth from the periphery to the center. Peculiarities of the vascularity of tumor models revealed by optoacoustic imaging were confirmed by fluorescent microscopy with FITC-dextran and morphological analysis. The obtained results may be important for the investigation of tumor development and for improvement of colon cancer treatment strategies.

光栅扫描光声血管造影在532 nm波长处的诊断深度为2 mm，横向分辨率为50μm，用于结肠癌模型中新血管生成的定量表征。比较了两种人类结肠腺癌（HT-29）和鼠结肠癌（CT26）在组织学和血管形成方面不同的肿瘤模型。两种起源的肿瘤均显示血管高低区域分布不均。快速生长的CT26肿瘤显示出从外周到中心的较高的血管生长速率。通过FITC-葡聚糖荧光显微镜和形态学分析证实了由光声成像揭示的肿瘤模型的血管特异性。