After informed consent we studied effect of pharmacological suppression of insulin secretion on tissue sensitivity to insulin in eight subjects with moderate obesity (relative body weight 133 +/- 6 per cent). Glucose tolerance (100 g oGTT) and insulin sensitivity were studied on two separate days before and after treatment with 500 mg diazoxide/d for a period of 4 d. Insulin sensitivity was determined by means of a 1-h priming dose-constant insulin infusion technique (two 30-min periods of 8 and 16 mU/kg, insulin MC-Actrapid, initiated by a start injection of 1 and 2 mU/kg, respectively). The relative decrease of plasma glucose and nonesterified fatty acid concentration at comparable steady-state insulin levels has been taken as an estimate of body sensitivity to insulin. Diazoxide treatment resulted in a significant suppression of glucose-stimulated early (insulin area 0-30 min) (P less than 0.05), late (insulin area 30-120 min) (P less than 0.01) and total insulin response (insulin area 0-120 min) (P less than 0.01) as well as a mild deterioration of glucose tolerance. Insulin sensitivity increased by about 50 per cent in comparison to the pretreatment value. Similarly, there was a marked increase of the antilipolytic effect of insulin after short-term treatment with diazoxide (P less than 0.05). The results support the concept that decreased sensitivity to insulin in obesity may be secondary due to hyperinsulinaemia.

中文翻译：

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胰岛素分泌的药理抑制作用对中度肥胖受试者的组织对胰岛素的敏感性的影响。

在知情同意后，我们研究了八位中度肥胖（相对体重133 +/- 6％）的药物抑制胰岛素分泌对组织对胰岛素敏感性的影响。在以500 mg diazoxide / d治疗4天之前和之后的两天，分别研究了葡萄糖耐量（100 g oGTT）和胰岛素敏感性。胰岛素敏感性通过1小时初免剂量恒定胰岛素输注技术确定（两次30分钟，分别为8和16 mU / kg，MC-Actrapid胰岛素，开始注射1和2 mU / kg，分别）。在相当的稳态胰岛素水平下，血浆葡萄糖和非酯化脂肪酸浓度的相对下降已被视为对胰岛素的身体敏感性的估计。二氮嗪治疗显着抑制了葡萄糖刺激的早期（胰岛素区域0-30分钟）（P小于0.05），晚期（胰岛素区域30-120分钟）（P小于0.01）和总胰岛素响应（胰岛素区域0） -120分钟）（P小于0.01）以及葡萄糖耐量的轻度降低。与预处理值相比，胰岛素敏感性提高了约50％。同样，在用二氮嗪短期治疗后，胰岛素的抗脂解作用显着增加（P小于0.05）。结果支持这样的概念，即肥胖引起的对胰岛素敏感性的降低可能是继发于高胰岛素血症。01）以及葡萄糖耐量的轻度下降。与预处理值相比，胰岛素敏感性提高了约50％。同样，在用二氮嗪短期治疗后，胰岛素的抗脂解作用显着增加（P小于0.05）。结果支持这样的概念，即肥胖引起的对胰岛素敏感性降低可能是继发于高胰岛素血症。01）以及葡萄糖耐量的轻度下降。与预处理值相比，胰岛素敏感性提高了约50％。同样，在用二氮嗪短期治疗后，胰岛素的抗脂解作用显着增加（P小于0.05）。结果支持这样的概念，即肥胖引起的对胰岛素敏感性的降低可能是继发于高胰岛素血症。