当前位置: X-MOL 学术Redox Rep. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Sicyos angulatus ameliorates acute liver injury by inhibiting oxidative stress via upregulation of anti-oxidant enzymes.
Redox Report ( IF 5.2 ) Pub Date : 2018-11-14 , DOI: 10.1080/13510002.2018.1546986
Hyun-Yong Kim 1, 2 , Jung-Ran Noh 1 , Sung-Je Moon 1 , Dong-Hee Choi 1 , Yong-Hoon Kim 1, 3 , Kyoung-Shim Kim 1, 3 , Hong-Sun Yook 2 , Jin-Pyo An 4 , Won-Keon Oh 4 , Jung Hwan Hwang 1, 3 , Chul-Ho Lee 1, 3
Affiliation  

Objective: We aimed to investigate the effect of Sicyos angulatus (SA) ethanolic extracts as antioxidants and potential treatments for liver disease.

Methods: To establish a mouse model of liver injury, C57BL/6 male mice were injected via the caudal vein with a single dose of concanavalin A (Con A, 15 mg kg−1). SA extracts were administered once by oral gavage 30 min before Con A injection.

Results: In vitro studies showed that SA decreased tert-butyl hydroperoxide (t-BHP)-induced reactive oxygen species (ROS) production. SA administration reduced plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as hepatic ROS levels, in a dose-dependent manner. Moreover, SA increased the activities of the hepatic antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase in a dose-dependent manner. Furthermore, SA treatment reduced pro-apoptotic protein levels. Con A-mediated cytosolic release of Smac/DIABLO and apoptosis-inducing factor (AIF), which are markers of necrosis, were dramatically decreased in HepG2 cells treated with SA.

Conclusion: SA ameliorated liver injury and might be a good strategy for the treatment of liver injury.



中文翻译:

角果西cyosos通过上调抗氧化酶抑制氧化应激,改善急性肝损伤。

目的:我们旨在研究安息香Sicyos angulatus,SA)乙醇提取物作为抗氧化剂的作用以及对肝脏疾病的潜在治疗方法。

方法:为建立肝损伤的小鼠模型,通过尾静脉向C57BL / 6雄性小鼠注射单剂量的伴刀豆球蛋白A(Con A,15 mg kg -1)。在Con A注射前30分钟,通过管饲法一次给予SA提取物。

结果: 体外研究表明,SA降低了丁基氢过氧化物(t -BHP)诱导的活性氧(ROS)的产生。SA给药以剂量依赖性方式降低血浆丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的水平以及肝ROS的水平。此外,SA以剂量依赖性方式增加了肝抗氧化酶超氧化物歧化酶,过氧化氢酶和谷胱甘肽过氧化物酶的活性。此外,SA治疗降低了促凋亡蛋白水平。在用SA处理的HepG2细胞中,Con A介导的Smac / DIABLO和细胞凋亡诱导因子(AIF)的细胞溶质释放(它们是坏死的标志物)显着减少。

结论: SA减轻了肝损伤,可能是治疗肝损伤的一种良好策略。

更新日期:2018-11-14
down
wechat
bug