Objective: We aimed to investigate the effect of Sicyos angulatus (SA) ethanolic extracts as antioxidants and potential treatments for liver disease.

Methods: To establish a mouse model of liver injury, C57BL/6 male mice were injected via the caudal vein with a single dose of concanavalin A (Con A, 15 mg kg⁻¹). SA extracts were administered once by oral gavage 30 min before Con A injection.

Results: In vitro studies showed that SA decreased tert-butyl hydroperoxide (t-BHP)-induced reactive oxygen species (ROS) production. SA administration reduced plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as hepatic ROS levels, in a dose-dependent manner. Moreover, SA increased the activities of the hepatic antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase in a dose-dependent manner. Furthermore, SA treatment reduced pro-apoptotic protein levels. Con A-mediated cytosolic release of Smac/DIABLO and apoptosis-inducing factor (AIF), which are markers of necrosis, were dramatically decreased in HepG2 cells treated with SA.

Conclusion: SA ameliorated liver injury and might be a good strategy for the treatment of liver injury.

目的：我们旨在研究安息香（Sicyos angulatus，SA）乙醇提取物作为抗氧化剂的作用以及对肝脏疾病的潜在治疗方法。

方法：为建立肝损伤的小鼠模型，通过尾静脉向C57BL / 6雄性小鼠注射单剂量的伴刀豆球蛋白A（Con A，15 mg kg ^-1）。在Con A注射前30分钟，通过管饲法一次给予SA提取物。

结果： 体外研究表明，SA降低了叔丁基氢过氧化物（t -BHP）诱导的活性氧（ROS）的产生。SA给药以剂量依赖性方式降低血浆丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的水平以及肝ROS的水平。此外，SA以剂量依赖性方式增加了肝抗氧化酶超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶的活性。此外，SA治疗降低了促凋亡蛋白水平。在用SA处理的HepG2细胞中，Con A介导的Smac / DIABLO和细胞凋亡诱导因子（AIF）的细胞溶质释放（它们是坏死的标志物）显着减少。

结论： SA减轻了肝损伤，可能是治疗肝损伤的一种良好策略。