We analyzed the frequency of single-nucleotide polymorphisms (SNPs) at positions -1053 (rs 2981572), 1380 (rs 2981573), 1462 (rs 2232360), and 3978 (rs 1518108) of the human interleukin-20 (IL-20) gene by tetraprimer ARMS-PCR method. A significant association between patients with psoriasis and the G allele at position -1053 (P<0.05) was established. The pairwise linkage disequilibrium (LD) matrix showed that the nearly complete LD was present within the polymorphisms at positions -1053, 1380, and 1462 of the IL-20 gene. We found that patients with plaque psoriasis had a higher frequency of the HT3 GAA haplotype (P<0.01, OR 2.341, 95% CI: 1.346-4.074) compared to the control group. Likewise, the HT3 GAA haplotype was associated with an increased risk of early-onset psoriasis (P<0.01, OR 2.305, 95% CI: 1.285-4.132), late onset of disease (P<0.01, OR 2.542, 95% CI: 1.266-5.102), familial psoriasis (P<0.02, OR 2.220, 95% CI: 1.249-3.945), and sporadic disease (P<0.01, OR 2.523, 95% CI: 1.390-4.580). Our data indicate that IL-20 gene polymorphisms should have a role in determining susceptibility to plaque-type psoriasis. The possible role of the studied SNPs in the regulation of the expression of IL-20 is unknown yet and needs further studies.

中文翻译：

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白细胞介素20基因中的多态性：与斑块型牛皮癣的关系。

我们分析了人类白介素20（IL-20）的位置-1053（rs 2981572），1380（rs 2981573），1462（rs 2232360）和3978（rs 1518108）的单核苷酸多态性（SNP）的频率。基因通过四引物ARMS-PCR方法鉴定。银屑病患者和-1053位的G等位基因之间存在显着相关性（P <0.05）。成对连锁不平衡（LD）矩阵显示，在IL-20基因的-1053、1380和1462位点的多态性内，存在几乎完整的LD。我们发现，与对照组相比，斑块状牛皮癣患者的HT3 GAA单倍型频率更高（P <0.01，或2.341，95％CI：1.346-4.074）。同样，HT3 GAA单倍型与早发性牛皮癣风险增加相关（P <0.01，或2.305，95％CI：1.285-4.132），疾病晚期发作（P <0.01，OR 2.542，95％CI：1.266-5.102），家族性牛皮癣（P <0.02，OR 2.220，95％CI：1.249-3.945）和散发性疾病（P <0.01，OR 2.523） ，95％CI：1.390-4.580）。我们的数据表明，IL-20基因多态性应在确定对斑块型牛皮癣的敏感性中起作用。尚不清楚所研究的SNP在调节IL-20表达中的可能作用，尚需进一步研究。