We have used the calcium indicator dye arsenazo III, together with a photodiode array, to record intracellular calcium changes simultaneously from all regions of individual guinea pig cerebellar Purkinje cells in slices. The optical signals, recorded with millisecond time resolution, are good indicators of calcium-dependent electrical events. For many cells the sensitivity of the recordings was high enough to detect signals from each array element without averaging. Consequently, it was possible to use these signals to follow the complex spatial and temporal patterns of plateau and spike potentials. Calcium entry corresponding to action potentials was detected from all parts of the dendritic field including the fine spiny branchlets, demonstrating that calcium action potentials spread over the entire arbor. Usually, the entire dendritic tree fired at once. But sometimes only restricted areas had signals at any one moment with transients detected in different regions at other times. In one cell, six separate zones were distinguished. These results show that calcium action potentials could be regenerative in some dendrites and could fail to propagate into others. Signals from plateau potentials were also detected from extensive areas in the dendritic field but were always smaller than those caused by a burst of action potentials.

中文翻译：

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豚鼠浦肯野细胞树突中钙依赖性电活动的时空分析。

我们已经使用了钙指示剂砷化偶氮III和光电二极管阵列，同时记录了切片中豚鼠小脑Purkinje细胞各个区域的细胞内钙变化。以毫秒时间分辨率记录的光信号，是钙依赖性电事件的良好指示。对于许多单元来说，记录的灵敏度足够高，可以不平均地检测每个阵列元件的信号。因此，可以使用这些信号来跟踪高原和尖峰电位的复杂空间和时间模式。从树突区域的所有部分（包括细刺小枝）中检测到与动作电位相对应的钙进入，这表明钙动作电位遍布整个乔木。通常，整个树突树立即被烧毁。但是有时，只有受限区域在任何时刻都有信号，而在其他时间在不同区域中检测到瞬变。在一个单元中，区分了六个单独的区域。这些结果表明钙动作电位可能在某些树突中再生，而可能无法传播到其他树突中。还从树突区域的广阔区域中检测到了来自高原电位的信号，但这些信号始终小于由动作电位爆发引起的信号。