The development of plant virus gene vectors for expression of foreign genes in plants provides attractive biotechnological tools to complement conventional breeding and transgenic methodology. The benefits of virus-based transient RNA and DNA replicons versus transgenic gene expression include rapid and convenient engineering coupled with flexibility for expeditious application in various plant species. These characteristics are especially advantageous when very high levels of gene expression are desired within a short time, although instability of the foreign gene in the viral genome can present some problems. The strategies that have been tested for foreign gene expression in various virus-based vectors include gene replacement, gene insertion, epitope presentation, use of virus controlled gene expression cassettes, and complementation. Recent reports of the utilization of virus vectors for foreign gene expression in fundamental research and biotechnology applications are discussed.

中文翻译：

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用于在植物中瞬时表达外源蛋白质的植物病毒基因载体。

用于在植物中表达外源基因的植物病毒基因载体的开发提供了有吸引力的生物技术工具，以补充常规育种和转基因方法。与转基因基因表达相比，基于病毒的瞬时RNA和DNA复制子的优势包括快速便捷的工程设计以及灵活的灵活性，可在各种植物中快速应用。这些特性在短时间内需要非常高水平的基因表达时特别有利，尽管病毒基因组中外源基因的不稳定性会带来一些问题。在各种基于病毒的载体中测试外源基因表达的策略包括基因替换，基因插入，表位呈递，使用病毒控制的基因表达盒和互补。