The increasing amount of available expressed gene sequence data makes whole-transcriptome analysis of certain crop species possible. Potato currently has the second largest number of publicly available expressed sequence tag (EST) sequences among the Solanaceae. Most of these ESTs, plus other proprietary sequences, were combined and used to generate a unigene assembly. The set of 246,182 sequences produced 46,345 unigenes, which were used to design a 44K 60-mer oligo array (Potato Oligo Chip Initiative: POCI). In this study, we attempt to identify genes controlling and driving the process of tuber initiation and growth by implementing large-scale transcriptional changes using the newly developed POCI array. Major gene expression profiles could be identified exhibiting differential expression at key developmental stages. These profiles were associated with functional roles in cell division and growth. A subset of genes involved in the regulation of the cell cycle, based on their Gene Ontology classification, exhibit a clear transient upregulation at tuber onset indicating increased cell division during these stages. The POCI array allows the study of potato gene expression on a much broader level than previously possible and will greatly enhance analysis of transcriptional control mechanisms in a wide range of potato research areas. POCI sequence and annotation data are publicly available through the POCI database ( http://pgrc.ipk-gatersleben.de/poci ).

中文翻译：

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驱动马铃薯块茎起始和生长的基因：使用 POCI 阵列基于转录变化进行鉴定。

越来越多的可用表达基因序列数据使得对某些作物物种的全转录组分析成为可能。马铃薯目前在茄科植物中拥有数量第二多的公开表达序列标签 (EST) 序列。这些 EST 中的大多数，加上其他专有序列，被组合并用于生成 unigene 组装。这组 246,182 个序列产生了 46,345 个 unigenes，用于设计 44K 60 聚体寡核苷酸阵列（Potato Oligo Chip Initiative：POCI）。在这项研究中，我们尝试通过使用新开发的 POCI 阵列实施大规模转录变化来识别控制和驱动块茎起始和生长过程的基因。可以鉴定出在关键发育阶段表现出差异表达的主要基因表达谱。这些特征与细胞分裂和生长中的功能作用有关。根据基因本体论分类，参与细胞周期调节的基因子集在块茎开始时表现出明显的瞬时上调，表明在这些阶段细胞分裂增加。POCI 阵列允许在比以前更广泛的水平上研究马铃薯基因表达，并将大大加强对广泛马铃薯研究领域的转录控制机制的分析。POCI 序列和注释数据可通过 POCI 数据库 (http://pgrc.ipk-gatersleben.de/poci) 公开获得。在块茎开始时表现出明显的瞬时上调，表明在这些阶段细胞分裂增加。POCI 阵列允许在比以前更广泛的水平上研究马铃薯基因表达，并将大大加强对广泛马铃薯研究领域的转录控制机制的分析。POCI 序列和注释数据可通过 POCI 数据库 (http://pgrc.ipk-gatersleben.de/poci) 公开获得。在块茎开始时表现出明显的瞬时上调，表明在这些阶段细胞分裂增加。POCI 阵列允许在比以前更广泛的水平上研究马铃薯基因表达，并将大大加强对广泛马铃薯研究领域的转录控制机制的分析。POCI 序列和注释数据可通过 POCI 数据库 (http://pgrc.ipk-gatersleben.de/poci) 公开获得。