A new scalable reactor was developed by applying a novel mixing principle that allows the large-scale cultivation of mammalian cells simply with surface aeration using air owing to increased liquid-gas transfer compared to standard stirred-tank bioreactors. In the cylindrical vessels (50 mL-1500 L) with a helical track attached to the inside wall, the liquid moved upward onto the track as the result of orbital shaking to increase the liquid-gas interface area significantly. This typically resulted in a 5-10-fold improvement in the volumetric mass transfer coefficient (k(L)a). In a 1500-L helical track vessel with a working volume of 1000 L, a k(L)a of 10h(-1) was obtained at a shaking speed of 39 rpm. Cultivations of CHO cells in a shaken 55-L helical track bioreactor resulted in improved cell growth profiles compared to control cultures in standard systems. These results demonstrated the possibility of using these new bioreactors at scales of 1000 L or more.

中文翻译：

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振动螺旋轨道生物反应器：通过空气表面曝气为体积高达 1000 升的哺乳动物细胞高密度培养物提供氧气

通过应用新型混合原理开发了一种新的可扩展反应器，与标准搅拌罐生物反应器相比，由于增加了液气转移，因此可以使用空气简单地通过表面曝气来大规模培养哺乳动物细胞。在内壁带有螺旋轨道的圆柱形容器（50 mL-1500 L）中，由于轨道振动，液体向上移动到轨道上，从而显着增加了液气界面面积。这通常会导致体积传质系数 (k(L)a) 提高 5-10 倍。在工作容积为 1000 L 的 1500-L 螺旋轨道容器中，在 39 rpm 的振动速度下获得了 10h(-1) 的 ak(L)a。与标准系统中的对照培养物相比，在摇动的 55-L 螺旋轨道生物反应器中培养 CHO 细胞可改善细胞生长情况。这些结果证明了在 1000 L 或更大的规模上使用这些新生物反应器的可能性。