Anaerobic degradation of p-xylene was studied with sulfate-reducing enrichment culture. The enrichment culture was established with sediment-free sulfate-reducing consortium on crude oil. The crude oil-degrading consortium prepared with marine sediment revealed that toluene, and xylenes among the fraction of alkylbenzene in the crude oil were consumed during the incubation. The PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene for the p-xylene degrading sulfate-reducing enrichment culture showed the presence of the single dominant DGGE band pXy-K-13 coupled with p-xylene consumption and sulfide production. Sequence analysis of the DGGE band revealed a close relationship between DGGE band pXy-K-13 and the previously described marine sulfate-reducing strain oXyS1 (similarity value, 99%), which grow anaerobically with o-xylene. These results suggest that microorganism corresponding to pXy-K-13 is an important sulfate-reducing bacterium to degrade p-xylene in the enrichment culture.

中文翻译：

---

在无沉淀物的硫酸盐减少浓缩培养中对二甲苯的厌氧降解。

用减少硫酸盐的富集培养研究了对二甲苯的厌氧降解。用无沉淀的硫酸盐还原财团在原油上建立了富集培养。由海洋沉积物制备​​的原油降解财团表明，在孵化过程中消耗了原油中烷基苯馏分中的甲苯和二甲苯。对二甲苯降解硫酸盐还原富集培养物的16S rRNA基因的PCR变性梯度凝胶电泳（DGGE）分析显示，存在单个显性DGGE谱带pXy-K-13以及对二甲苯的消耗和硫化物的产生。DGGE谱带的序列分析显示DGGE谱带pXy-K-13与先前描述的减少海洋硫酸盐还原的菌株oXyS1（相似度为99％）之间存在密切关系，与邻二甲苯厌氧生长。这些结果表明，对应于pXy-K-13的微生物是富集培养物中降解对二甲苯的重要的硫酸盐还原细菌。