Arsenic-induced tissue damage is a major concern to the human population. An impaired antioxidant defense mechanism followed by oxidative stress is the major cause of arsenic-induced toxicity, which can lead to reproductive failure. The present study was carried out to investigate the preventive role of arjunolic acid, a triterpenoid saponin isolated from the bark of Terminalia arjuna, against arsenic-induced testicular damage in mice. Administration of arsenic (in the form of sodium arsenite, NaAsO(2), at a dose of 10 mg/kg body weight) for 2 days significantly decreased the intracellular antioxidant power, the activities of the antioxidant enzymes, as well as the levels of cellular metabolites. In addition, arsenic intoxication enhanced testicular arsenic content, lipid peroxidation, protein carbonylation and the level of glutathione disulfide (GSSG). Exposure to arsenic also caused significant degeneration of the seminiferous tubules with necrosis and defoliation of spermatocytes. Pretreatment with arjunolic acid at a dose of 20 mg/kg body weight for 4 days could prevent the arsenic-induced testicular oxidative stress and injury to the histological structures of the testes. Arjunolic acid had free radical scavenging activity in a cell-free system and antioxidant power in vivo. In summary, the results suggest that the chemopreventive role of arjunolic acid against arsenic-induced testicular toxicity may be due to its intrinsic antioxidant property.

中文翻译：

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砷酸对砷诱导的睾丸氧化应激的保护作用。

砷引起的组织损伤是人类的主要关切。氧化应激后抗氧化剂防御机制受损是砷诱导的毒性的主要原因，这可导致生殖衰竭。本研究旨在研究从榄仁木树皮中分离出的三萜类皂苷-箭果酸对砷诱导的小鼠睾丸损伤的预防作用。连续2天施用砷（以砷酸钠NaAsO（2）的形式，剂量为10 mg / kg体重）显着降低细胞内抗氧化能力，抗氧化酶的活性以及细胞代谢产物。此外，砷中毒会增加睾丸中的砷含量，脂质过氧化，蛋白羰基化和谷胱甘肽二硫键（GSSG）的水平。接触砷还导致生精小管明显变性，并伴有精母细胞坏死和脱叶。以20 mg / kg体重的剂量进行4天的预处理可以预防砷诱导的睾丸氧化应激和对睾丸组织结构的伤害。阿苏酸在无细胞系统中具有清除自由基的活性，在体内具有抗氧化能力。总而言之，该结果表明，由于其固有的抗氧化特性，因此，果酸对砷诱导的睾丸毒性具有化学预防作用。以20 mg / kg体重的剂量进行4天的预处理可以预防砷诱导的睾丸氧化应激和对睾丸组织结构的伤害。阿苏酸在无细胞系统中具有清除自由基的活性，在体内具有抗氧化能力。总而言之，该结果表明，由于其固有的抗氧化特性，因此，果酸对砷诱导的睾丸毒性具有化学预防作用。以20 mg / kg体重的剂量进行4天的预处理可以预防砷诱导的睾丸氧化应激和对睾丸组织结构的伤害。阿苏酸在无细胞系统中具有清除自由基的活性，在体内具有抗氧化能力。总而言之，该结果表明，由于其固有的抗氧化特性，因此，果酸对砷诱导的睾丸毒性具有化学预防作用。