Mixed bacterial cultures aerobically transformed decalin (decahydronaphthalene) dissolved in an immiscible carrier phase (heptamethylnonane; HMN) in liquid medium. Conversion was enhanced in the presence of decane, a readily degraded n-alkane, and/or HMN. Four Rhodococcus spp. isolates purified from one of the mixed cultures were active against decalin in the presence of n-decane, but their ability to use decalin as a sole carbon source for growth could not be sustained. Isolate Iso 1a oxidized decalin under co-metabolic conditions with decane vapours as the primary carbon source. Mass spectrometry and comparison to authentic standards showed that the oxidized products of decalin biotransformation were 2-decahydronaphthol and 2-decalone. Some evidence of ring-opening was obtained, but the possible ring-opened product was not definitively identified. These results are consistent with co-metabolic oxidation of decalin by enzymes active toward n-alkanes.

中文翻译：

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Rhodococcus spp对十氢化萘（十氢萘）进行有氧生物转化。

混合细菌培养物需氧转化为溶解在液体介质中不混溶的载体相（庚甲基壬烷； HMN）中的十氢化萘（十氢化萘）。在癸烷，易降解的正构烷烃和/或HMN的存在下，转化率得以提高。四个红球菌属。从混合培养物中的一种纯化得到的分离株在正癸烷的存在下对十氢化萘具有活性，但不能维持其将十氢化萘用作唯一碳源进行生长的能力。在癸烷蒸气为主要碳源的共代谢条件下分离Iso 1a氧化的十氢化萘。质谱分析和与真实标准品的比较表明，萘烷生物转化的氧化产物为2-癸氢萘酚和2-decalone。获得了一些开环的证据，但尚未确定可能的开环产品。这些结果与由对正构烷烃有活性的酶对十氢化萘的共代谢氧化相一致。