The untanned proteinaceous tannery solid waste, the animal fleshing (ANFL), was used as substrate in the treatment process (hydrolysis and fermentation) involving Synergistes sp. The nonionic surfactant (Tween 80) was evaluated for its ability to influence on microbial growth and enzyme activity in the hydrolysis and fermentation of ANFL. The addition of Tween 80 in the process significantly increased the activities of hydrolytic and fermentative enzymes like protease (338-360 Um l(-1)) and deaminase (187-206 Um l(-1)) compared to that of control (protease 195-220 Um l(-1) and deaminase 70-83 Um l(-1)). The total viable bacterial count was increased more than twofold, compared to the control in the presence of 0.15% Tween 80. The ANFL fermentation and formation of other metabolites were evidenced by Gas Chromatography and Mass Spectroscopy (GC-MS), Proton Nuclear Magnetic Resonance spectroscopy ((1)H NMR) and Fourier transform infra red spectroscopy (FT-IR). The breakdown of fibrillar proteins in ANFL was confirmed by the scanning electron microscopy (SEM) and the transmission electron microscopy (TEM).

中文翻译：

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非离子表面活性剂对富含蛋白质的制革厂固体废物水解和发酵的影响。

在涉及Synergistes sp。的处理过程（水解和发酵）中，将未鞣制的蛋白质制革厂固体废物（动物肉（ANFL））用作基质。评价了非离子表面活性剂（Tween 80）在ANFL水解和发酵过程中影响微生物生长和酶活性的能力。与对照（蛋白酶）相比，在此过程中添加Tween 80可显着提高水解和发酵酶（如蛋白酶（338-360 Um l（-1））和脱氨酶（187-206 Um l（-1））的活性。 195-220 Um l（-1）和脱氨酶70-83 Um l（-1））。与存在0.15％Tween 80的对照组相比，总的活细菌计数增加了两倍以上。ANFL发酵和其他代谢物的形成通过气相色谱和质谱（GC-MS）进行了证明，质子核磁共振波谱（（1）H NMR）和傅立叶变换红外光谱（FT-IR）。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）证实ANFL中纤维状蛋白的分解。