To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappaB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.

中文翻译：

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细胞培养条件增强了从乳腺癌患者和健康受试者中分离出的外周血白细胞对电离辐射的响应差异。

为了比较电离辐射对乳腺癌患者和健康受试者白细胞的离体作用，其NF-κB和抗氧化酶的含量分别为锰超氧化物歧化酶（Mn-SOD），铜锌超氧化物歧化酶（CuZn-进行了SOD和过氧化氢酶（CAT）结合CD4 +淋巴细胞的流式细胞术分析。与健康受试者相比，乳腺癌研究组中的Mn-SOD蛋白水平在放疗前（P <0.001）和放疗后（P <0.001）均显着增加。并行测量表明，与健康受试者相比，乳腺癌研究组中放射后的CAT蛋白水平显着更高（2 Gy [P <0.001]和9 Gy [P <0.05]）。尽管乳腺癌患者血液中的淋巴细胞初始数量与健康受试者无差异，但放射前后凋亡的CD4 +细胞百分比显着降低（P <0.001），这表明细胞培养条件诱导了CD4 +细胞的放射抗性。乳腺癌患者的血液。这项当前研究中提供的数据表明，外周血白细胞的简短体外培养可增强由乳腺癌肿瘤引起的氧化应激。