Thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using long-chain hydrocarbons (waste car engine oil, base oil or the c-alkane fraction of base oil) as the sole carbon and energy source. A phylogenetic tree of the isolates constructed using their 16S rDNA sequences revealed that the isolates were divided into six genera plus one family (Acinetobacter, Rhodococcus, Gordonia, Pseudomonas, Ralstonia, Bacillus and Alcaligenaceae, respectively). Furthermore, most of the isolates (27 of 36) were classified into the genera Acinetobacter, Rhodococcus or Gordonia. The hydrocarbon-degradation similarity in each strain was confirmed by the 2,6-dichlorophenol indophenol (2,6-DCPIP) assay. Isolates belonging to the genus Acinetobacter degraded long-chain normal alkanes (n-alkanes) but did not degrade short-chain n-alkanes or cyclic alkanes (c-alkanes), while isolates belonging to the genera Rhodococcus and Gordonia degraded both long-chain n-alkanes and c-alkanes.

中文翻译：

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长链降解碳氢化合物细菌的系统发育分析和通过2,6-DCPIP分析对其碳氢化合物的降解进行评估。

使用长链碳氢化合物（废发动机油，基础油或基础油的正构烷烃馏分）作为唯一的碳源和能源，从自然环境中分离出了降解长链碳氢化合物的36种细菌。用其16S rDNA序列构建的分离株的系统进化树显示，分离株分为6个属和1个科（不动杆菌，红球菌，戈多尼亚，假单胞菌，罗氏菌，芽孢杆菌和拟南芥科）。此外，大多数分离株（36个中的27个）被分为不动杆菌属，红球菌属或戈登尼亚属。通过2，6-二氯苯酚吲哚酚（2，6-DCPIP）分析证实了每个菌株中的烃降解相似性。