Over the last 15 years, the ability of mass spectrometry to analyze complex peptide mixtures and identify individual species has provided unprecedented insights into the repertoire of peptide antigens displayed by MHC molecules and recognized by T lymphocytes. These include: understanding the peptide binding specificity of MHC molecules; understanding of roles of different intracellular components of the antigen processing pathways in determining the peptide display; and identification of a large number of individual peptide antigens associated with infectious diseases, cancer, and transplant rejection that have provided the basis for new immunologically based therapies. This review will summarize the impact that the application of mass spectrometry has had on these advances, with particular attention to the contributions of Professor Donald Hunt and members of his laboratory, and point out the opportunities for future work.

中文翻译：

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质谱对理解 T 淋巴细胞免疫识别的贡献

在过去的 15 年中，质谱分析复杂肽混合物和识别单个物种的能力为 MHC 分子展示和 T 淋巴细胞识别的肽抗原库提供了前所未有的见解。这些包括：了解 MHC 分子的肽结合特异性；了解抗原加工途径的不同细胞内成分在决定肽展示中的作用；和鉴定大量与传染病、癌症和移植排斥相关的个体肽抗原，这些抗原为新的基于免疫学的疗法提供了基础。本综述将总结质谱的应用对这些进步的影响，