In this study we used the assimilation of isotope labeled CO(2) to measure the substrate preferences by two different bioaugmentation mixtures proposed for bioremediation of diesel oil contamination. All active microorganisms assimilate CO(2) in various carboxylation processes involved in growth. The CO(2) assimilation by the two mixtures was measured upon addition of glucose, diesel oil or specific compounds present in diesel oil (naphthalene, toluene, hexadecane, and octane). It was shown that within short term incubations with diesel oil (<5 h), one bioaugmentation mixture was superior to the other regarding the assimilation of CO(2). This observation was confirmed in a labor-intensive long term microcosm study (60 days). The applied method open various possibilities for fast pre-testing of substrate-preferences by microbial-bioaugmentation mixtures without microcosm experiments, on-site tests, and complicated chemical analysis. This study also demonstrates the possibility to obtain further information on the substrate preferences at a single cell level of phylogenetically defined microbial subgroups in bioaugmentation mixtures, based on combined analyses of microautoradiography and fluorescence in situ hybridization.

中文翻译：

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提出了用于生物增强的微生物联合体的个体成员测量底物偏好的方法。

在这项研究中，我们使用了同位素标记为CO（2）的同化作用，通过两种不同的生物强化混合物提出了对柴油污染的生物修复方法来测量底物的偏爱。所有活跃的微生物同化参与生长的各种羧化过程中的CO（2）。在添加葡萄糖，柴油或柴油中存在的特定化合物（萘，甲苯，十六烷和辛烷）后，测量了两种混合物对CO（2）的同化作用。结果表明，在与柴油（<5小时）的短期孵育中，一种生物强化混合物在同化CO（2）方面优于另一种。一项劳动密集型长期微观研究（60天）证实了这一观察结果。应用的方法为通过微生物-生物强化混合物快速进行底物偏好的快速测试提供了各种可能性，而无需进行微观实验，现场测试和复杂的化学分析。这项研究还表明，基于微放射自显影和荧光原位杂交的综合分析，可以在生物强化混合物中的系统发育定义的微生物亚组的单个细胞水平上获得有关底物偏好的更多信息。