Reports of ivermectin resistance in scabies mites raise concerns regarding the sustainability of mass intervention programs for scabies worldwide and for the treatment of crusted scabies. Ligand gated ion channels (LGICs) are the primary targets of ivermectin in invertebrates. We report the molecular characterisation of SsCl--a novel LGIC from Sarcoptes scabiei var. hominis. While SsCl shows sequence similarity to other LGICs, phylogenetic analysis does not suggest strong homology to conventional glutamate, histamine or GABA gated channels. Instead, it is most similar to Drosophila pH-sensitive and group 1 clades. When expressed in Xenopus oocytes, SsCl forms a homomeric, pH-gated chloride channel that is irreversibly activated by ivermectin. These results provide the first confirmation that this group of LGIC exists in arachnids, and suggest that SsCl may be an in vivo target of ivermectin in S. scabiei.

中文翻译：

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pH值门控的Sarcoptes scabiei的氯离子通道的分子表征。

关于sc螨中伊维菌素耐药性的报道引起了人们对全球sc疮和结sc疮治疗大规模干预计划的可持续性的担忧。配体门离子通道（LGIC）是伊维菌素在无脊椎动物中的主要靶标。我们报告了SsCl的分子表征-一种来自Sarcoptes scabiei var的新型LGIC。原始人 虽然SsCl显示与其他LGIC的序列相似性，但系统进化分析并未显示与常规谷氨酸，组胺或GABA门控通道的强同源性。相反，它与果蝇对pH敏感的1类进化枝最为相似。当在非洲爪蟾卵母细胞中表达时，SsCl形成一个同构的，pH门控的氯离子通道，该通道被伊维菌素不可逆地激活。这些结果首次证实了这一类LGIC存在于蜘蛛纲动物中，