Information on the olfactory system in antennae of Geometridae moths is very limited, and odorant-binding proteins (OBPs) working as transporters of lipophilic odors have not been identified. In the first investigation on this family of insects, we examined antennal OBPs of the Japanese giant looper, Ascotis selenaria cretacea. RT-PCR experiments using several pairs of degenerate primers designed from known cDNA sequences encoding lepidopteran OBPs successfully amplified partial sequences of two pheromone-binding proteins (PBPs), named AscrPBP1 and AscrPBP2 in reference to their corresponding nucleotide sequence homologies with other PBPs. Using 5'- and 3'-rapid amplification of cDNA end strategies, a cDNA clone for AscrPBP1 encoding a protein of 141 amino acids was isolated. Western blotting with the antiserum against recombinant AscrPBP1 overexpressed in Escherichia coli showed that the AscrPBP1 gene was more strongly expressed in male antennae than in female antennae. Furthermore, natural AscrPBP1was isolated by immunoprecipitation with the antiserum, and its binding ability was evaluated by using synthetic sex pheromonal compounds with a C(19) chain. The result indicated that AscrPBP1 bound not only the pheromone components, 3,6,9-nonadecatriene and its 3,4-epoxy derivative, but also unnatural 6,7- and 9,10-epoxy derivatives. While no general odorant-binding proteins (GOBPs) were amplified in the RT-PCR experiments, two antisera prepared from GOBP1 and GOBP2 of Bombyx mori suggested the occurrence of at least two GOBPs in the A. s. cretacea antennae.

中文翻译：

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分析一种几何形状的物种（Ascotis selenaria cretacea）触角中的气味结合蛋白，该触角产生鳞翅目II型性信息素成分。

关于尺metri蛾的触角中的嗅觉系统的信息非常有限，还没有发现用作亲脂性气味转运体的气味结合蛋白（OBP）。在对该昆虫家族的首次调查中，我们检查了日本巨型弯嘴白粉虱（Ascotis selenaria cretacea）的触角OBP。使用几对简并引物进行RT-PCR实验，从已知的编码鳞翅目OBP的cDNA序列设计引物，成功地扩增了两个信息素结合蛋白（PBP）的部分序列，称为AscrPBP1和AscrPBP2，参考了它们与其他PBP的相应核苷酸序列同源性。使用cDNA末端策略的5'-和3'-快速扩增，分离了编码141个氨基酸的蛋白质的AscrPBP1的cDNA克隆。用在大肠杆菌中过表达的针对重组AscrPBP1的抗血清进行的蛋白质印迹显示，AscrPBP1基因在雄性触角中的表达比雌性触角中的表达强。此外，通过与抗血清免疫沉淀分离了天然AscrPBP1，并通过使用具有C（19）链的合成性信息素化合物评估了其结合能力。结果表明，AscrPBP1不仅结合信息素成分3,6,9-十九碳三烯及其3,4-环氧衍生物，而且还结合非天然的6,7-和9,10-环氧衍生物。尽管在RT-PCR实验中未扩增任何普通的气味结合蛋白（GOBP），但从家蚕的GOBP1和GOBP2制备的两种抗血清表明在拟南芥中至少存在两个GOBP。白垩纪触角。