Axonal guidance signals are transduced through growth cone surface receptors to the interior leading to changes of actin dynamics and actin binding proteins, which are critical in determining the outcome of actin cytoskeleton reorganization. We report here the characterization of the Drosophila actin binding protein abLIM/Unc-115 homolog Dunc-115 and its role in the nervous system. Three Dunc-115 isoforms are identified as Dunc-115L, M and S, respectively. While Dunc-115L is a canonical homolog of Unc-115 with four LIM domains and one villin headpiece domain, Dunc-115M and S are novel isoforms without counterparts in other species. Our molecular modeling shows Dunc-115L is likely to bind to actin. Mutant analysis reveals that Dunc-115 is involved in axonal projection in both the visual and central nervous system.

中文翻译：

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果蝇基因dunc-115在神经系统中的作用。

轴突引导信号通过生长锥表面受体转导至内部，导致肌动蛋白动力学和肌动蛋白结合蛋白发生变化，这对确定肌动蛋白细胞骨架重组的结果至关重要。我们在这里报告果蝇肌动蛋白结合蛋白abLIM / Unc-115同源物Dunc-115的表征及其在神经系统中的作用。三个Dunc-115同工型分别被标识为Dunc-115L，M和S。Dunc-115L是Unc-115的典型同系物，具有四个LIM域和一个villin头域，而Dunc-115M和S是新颖的同种型，在其他物种中没有类似物。我们的分子模型表明Dunc-115L可能与肌动蛋白结合。突变分析表明，Dunc-115参与了视觉和中枢神经系统的轴突投射。