In 1962, I proposed a model for integration of lambda prophage into the bacterial chromosome. The model postulated two steps (i) circularization of the linear DNA molecule that had been injected into the cell from the phage particle; (ii) reciprocal recombination between phage and bacterial DNA at specific sites on both partners. This resulted in a cyclic permutation of gene order going from phage to prophage. This contrasted with integration models current at the time, which postulated that the prophage was not inserted into the continuity of the chromosome but rather laterally attached or synapsed with it. This chapter summarizes some of the steps leading up to the model including especially the genetic characterization of specialized transducing phages (lambdagal) by recombinational rescue of conditionally lethal mutations. The serendipitous discovery of the conditional lethals is also described.

中文翻译：

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噬菌体整合和染色体结构。个人历史。

1962年，我提出了一个将λ噬菌体整合入细菌染色体的模型。该模型假定了两个步骤（i）将已经从噬菌体颗粒注入细胞的线性DNA分子环化；（ii）噬菌体与细菌DNA在两个伴侣的特定位点之间进行相互重组。这导致基因顺序从噬菌体到噬菌体的循环排列。这与当时的整合模型形成对比，后者假定该噬菌体未插入到染色体的连续性中，而是侧向附着或突触了它。本章总结了导致该模型的一些步骤，特别是通过重组挽救有条件致死性突变的专门转导噬菌体（lambdagal）的遗传表征。