Signaling pathways regulating proliferation, differentiation, and inflammation are commonly mediated through protein-protein interactions as well as reversible modification (e.g., phosphorylation) of proteins. To facilitate the study of regulated protein-protein interactions in cells and living animals, new imaging tools, many based on optical signals and capable of quantifying protein interactions in vivo, have advanced the study of induced protein interactions and their modification, as well as accelerated the rate of acquisition of these data. In particular, use of protein fragment complementation as a reporter strategy can accurately and rapidly dissect protein interactions with a variety of readouts, including absorbance, fluorescence, and bioluminescence. This review focuses on the development and validation of bioluminescent protein fragment complementation reporters that use either Renilla luciferase or firefly luciferase in vivo. Enhanced luciferase complementation provides a platform for near real-time detection and characterization of regulated and small-molecule-induced protein-protein interactions in intact cells and living animals and enables a wide range of novel applications in drug discovery, chemical genetics, and proteomics research.

中文翻译：

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与遗传编码的记者体内成像的蛋白质-蛋白质相互作用的当前状态。

调节增殖，分化和炎症的信号传导途径通常是通过蛋白质间相互作用以及蛋白质的可逆修饰（例如磷酸化）介导的。为了促进对细胞和活体动物中调节的蛋白质-蛋白质相互作用的研究，许多基于光信号并且能够在体内定量蛋白质相互作用的新型成像工具，已经促进了诱导蛋白质相互作用及其修饰的研究，并加速了这些数据的获取率。特别是，使用蛋白质片段互补作为报告基因策略可以准确快速地分析具有各种读数（包括吸光度，荧光和生物发光）的蛋白质相互作用。这篇综述的重点是在体内使用海肾荧光素酶或萤火虫荧光素酶的生物发光蛋白片段互补报告基因的开发和验证。增强的萤光素酶互补作用提供了一个平台，可用于近实时检测和表征完整细胞和活体动物中受调节的和小分子诱导的蛋白质-蛋白质相互作用，并在药物发现，化学遗传学和蛋白质组学研究中广泛应用。