In this paper, centrifuge-based microfluidic platforms are reviewed and compared with other popular microfluidic propulsion methods. The underlying physical principles of centrifugal pumping in microfluidic systems are presented and the various centrifuge fluidic functions, such as valving, decanting, calibration, mixing, metering, heating, sample splitting, and separation, are introduced. Those fluidic functions have been combined with analytical measurement techniques, such as optical imaging, absorbance, and fluorescence spectroscopy and mass spectrometry, to make the centrifugal platform a powerful solution for medical and clinical diagnostics and high throughput screening (HTS) in drug discovery. Applications of a compact disc (CD)-based centrifuge platform analyzed in this review include two-point calibration of an optode-based ion sensor, an automated immunoassay platform, multiple parallel screening assays, and cellular-based assays. The use of modified commercial CD drives for high-resolution optical imaging is discussed as well. From a broader perspective, we compare technical barriers involved in applying microfluidics for sensing and diagnostic use and applying such techniques to HTS. The latter poses less challenges and explains why HTS products based on a CD fluidic platform are already commercially available, whereas we might have to wait longer to see commercial CD-based diagnostics.

中文翻译：

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CD上的实验室。

在本文中，回顾了基于离心的微流体平台并将其与其他流行的微流体推进方法进行了比较。介绍了微流体系统中离心泵的基本物理原理，并介绍了各种离心机流体功能，例如阀门，倾析，校准，混合，计量，加热，样品分裂和分离。这些流体功能已与分析测量技术（例如光学成像，吸光度以及荧光光谱和质谱分析）相结合，使离心平台成为用于药物发现中医学和临床诊断以及高通量筛选（HTS）的强大解决方案。本文中分析的基于光盘（CD）的离心机平台的应用包括基于光电二极管的离子传感器的两点校准，自动免疫测定平台，多个平行筛选测定和基于细胞的测定。还讨论了将修改后的商用CD驱动器用于高分辨率光学成像的用途。从更广泛的角度来看，我们比较了将微流体用于传感和诊断用途以及将此类技术应用于HTS所涉及的技术障碍。后者所带来的挑战更少，并解释了为什么基于CD流体平台的HTS产品已经可以在市场上买到，而我们可能不得不等待更长的时间才能看到基于CD的商业诊断程序。我们比较了将微流体用于传感和诊断用途并将此类技术应用于HTS涉及的技术障碍。后者所带来的挑战更少，并解释了为什么基于CD流体平台的HTS产品已经可以在市场上买到，而我们可能不得不等待更长的时间才能看到基于CD的商业诊断程序。我们比较了将微流体用于传感和诊断用途并将此类技术应用于HTS涉及的技术障碍。后者所带来的挑战更少，并解释了为什么基于CD流体平台的HTS产品已经可以在市场上买到，而我们可能不得不等待更长的时间才能看到基于CD的商业诊断程序。