当前位置: X-MOL 学术STEM CELLS › 论文详情
Analysis of the cell cycle of late erythroid progenitor cells by sedimentation at unit gravity.
STEM CELLS ( IF 5.614 ) Pub Date : 1981-01-01
G Wagemaker,T P Visser

Approximately 70% of the late erythroid progenitor cells (E-CFU) which are present in normal bone marrow are killed by exposure to high specific activity tritiated thymidine (3H-TdR) in vitro or to hydroxyurea in vivo, indicating that a high proportion of these cells synthesize DNA. Their cell cycle was further analyzed by sedimentation at unit gravity. The modal sedimentation rate of 7.4 mm/h appeared to correspond to the fraction that is killed by 3H-TdR. Cells sedimenting at 6.0 and 9.5 mm/h were not susceptible to kill by 3H-TdR and correspond to cells in, respectively, G1 and G2/M. Based on a buoyant density of 1.077 g/cm3, the modal diameter of cells in G1 was calculated to be 8.3 micron, in S 9.2 micron and in G2/M 10.5 micron. The E-CFU population appeared to be composed of about 18% G1 cells, about 70% S phase cells and about 12% G2/M cells. The surviving fraction of E-CFU 2 h after intraperitoneal administration of 1 g/kg hydroxyurea was identified as being mainly a synchronous population in early S phase. The data are best explained by a short duration of G1 and G2/M phases.
更新日期:2019-11-01

 

全部期刊列表>>
宅家赢大奖
向世界展示您的会议墙报和演示文稿
全球疫情及响应:BMC Medicine专题征稿
新版X-MOL期刊搜索和高级搜索功能介绍
化学材料学全球高引用
ACS材料视界
x-mol收录
自然科研论文编辑服务
南方科技大学
南方科技大学
西湖大学
中国科学院长春应化所于聪-4-8
复旦大学
课题组网站
X-MOL
深圳大学二维材料实验室张晗
中山大学化学工程与技术学院
试剂库存
天合科研
down
wechat
bug