We examined the optimal conditions for maintaining normal human hematopoietic stem cells without cryopreservation. The effects of serum, HEPES, CPD and storage temperature were studied. Stem cell integrity (CFU-c, CFU-e, and BFU-e) was optimally maintained when the bone marrow cells were suspended in a medium containing 40% fetal calf serum, 25 mM HEPES, and 12.28% CPD and maintained at 4 degrees C. For example, under these storage conditions the recovery of functionally intact early erythroid stem cells, BFU-e, was 84% after 2 days, 44% after 4 days and 12% after 7 days. These results indicate that non-frozen short-term storage of human bone marrow may be of clinical utility in bone marrow transplantation.

中文翻译：

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非冷冻保存的来自正常人骨髓的造血干细胞。

我们检查了无需冷冻保存即可维持正常人造血干细胞的最佳条件。研究了血清，HEPES，CPD和储存温度的影响。当将骨髓细胞悬浮在含有40％胎牛血清，25 mM HEPES和12.28％CPD的培养基中并维持在4度时，可以最佳地维持干细胞完整性（CFU-c，CFU-e和BFU-e）。例如，在这些储存条件下，功能完整的早期红系干细胞BFU-e的回收率在2天后为84％，在4天后为44％，在7天后为12％。这些结果表明，非冷冻人骨髓的短期储存可能在骨髓移植中具有临床应用价值。