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Long-distance transportation of specimens for human tumor cloning.
STEM CELLS ( IF 4.0 ) Pub Date : 1982-01-01
D D Von Hoff , K D Swenerton , L Hetanen , E Gafney , S Rivkin , S Schuchart , C A Coltman

52 malignant effusions and 22 solid tumors were shipped from hospitals farther than 1,500 mi away to a central laboratory for culture in a human tumor-cloning system. Overall, 56% of the malignant effusions and 50% of the solid tumor specimens formed greater than or equal to 30 colonies in control plates (per 500,000 nucleated cells plated). This experience compares favorably to the percent of evaluable specimens which are cultured in the same institution which they are harvested. We conclude that with appropriate preparation, both malignant effusions and solid human tumors can be shipped to a central cloning laboratory with reasonable percentages of growth.

中文翻译:

标本的远距离运输,用于人类肿瘤克隆。

52例恶性积液和22例实体瘤从距离1,500英里远的医院运到了一个中心实验室,用于在人类肿瘤克隆系统中进行培养。总体而言,在对照平板中(每接种500,000个有核细胞),有56%的恶性积液和50%的实体瘤样本形成大于或等于30个菌落。与在相同采摘机构中培养的可评估标本的百分比相比,这种经验具有优势。我们得出的结论是,通过适当的准备,恶性积液和实体人类肿瘤都可以被运送到具有合理增长百分比的中央克隆实验室。
更新日期:2019-11-01
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