Inbred mouse strains C57BL/10Sn (Sn) and C57BL/10ScN (ScN) differ in response of their hematopoietic system to injection of lipopolysaccharide-W (LPS-W) in a manner similar to that observed for the LPS unresponsive C3H/HeJ and the paired responsive C3H/HeN strain mice. Responses of endogenous (E-CFU) stem cells as well as bone marrow and spleen-derived exogenous (CFU-s) stem cells, granulocyte-macrophage (GM-CFC) and macrophage (M-CFC) colony-forming cells were determined for Sn and ScN strain mice following an intraperitoneal injection of 10 micrograms LPS-W. Sn strain mice responded characteristically in terms of every parameter measured. Marrow-derived parameters reflected release of nucleated cells and early decrease of CFU-s, GM-CFC, and M-CFC followed by return toward control values. Peak splenic responses were observed within 4-5 days, whereas E-CFU increased significantly within 24 h after LPS-W. These responses were in marked contrast to those observed for the ScN strain mice, which were relatively unresponsive in terms of each parameter measured after LPS-W injection. These results show that the phenotypic expression of the defective LPS locus recently described in the ScN strain mice extends to those cells that control the response of the hematopoietic system to LPS, and is similar in every respect to those responses observed for the mutant LPS defective, C3H/HeJ strain mice.

中文翻译：

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C57BL / 10Sn和C57BL / 10ScN品系小鼠对脂多糖的造血反应。

自交系小鼠品系C57BL / 10Sn（Sn）和C57BL / 10ScN（ScN）在造血系统对注射脂多糖-W（LPS-W）的反应方面有所不同，其方式类似于对LPS无反应的C3H / HeJ和配对的响应C3H / HeN品系小鼠。确定内源性（E-CFU）干细胞以及骨髓和脾源性外源性（CFU-s）干细胞，粒细胞巨噬细胞（GM-CFC）和巨噬细胞（M-CFC）集落形成细胞的反应腹膜内注射10毫克LPS-W后的Sn和ScN品系小鼠。Sn品系小鼠在每个测量参数方面均具有特征性反应。骨髓来源的参数反映了有核细胞的释放和CFU-s，GM-CFC和M-CFC的早期减少，然后又回到控制值。在4-5天内观察到脾脏反应高峰，而LPS-W后24小时内E-CFU显着增加。这些反应与ScN品系小鼠观察到的反应形成鲜明对比，ScN品系小鼠在LPS-W注射后测量的每个参数方面相对没有反应。这些结果表明，最近在ScN品系小鼠中描述的缺陷LPS基因座的表型表达延伸到控制造血系统对LPS响应的那些细胞，并且在各个方面都与对突变LPS缺陷观察到的响应相似， C3H / HeJ品系小鼠。