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Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity
New Biotechnology ( IF 4.5 ) Pub Date : 2012-01-01 , DOI: 10.1016/j.nbt.2011.06.015
Hendrik Krauter 1 , Thomas Willke , Klaus-Dieter Vorlop
Affiliation  

3-hydroxypropionaldehyde (3HPA) is a promising versatile substance derived from the renewable feedstock glycerol. It is a product of glycerol metabolism in Lactobacillus reuteri. Because of toxic effects, the biotechnological production is poor. In this work the biocatalyst lifetime and product formation could be drastically increased. In the established two-step process already applied, cells are grown in the first step under anaerobic conditions, and in the second step the immobilised or suspended biocatalyst is used for 3HPA-production under strict anaerobic conditions. In the first step it was possible to reach a biomass concentration of 5.5g CDW/L (OD(600)≈23.4). In the second step, normally, 3HPA accumulates to a toxic concentration and the reaction stops in less than 60min because of the interaction of 3HPA with cell components. To prevent this, the toxic product is bound to the newly found scavenger carbohydrazide to form the hydrazone. For the first time it was possible to recycle the immobilised biocatalyst for at least ten cycles (overall life time>33hours) in a repeated batch biotransformation with an overall production of 67g 3HPA. The optimal pH-value was between 6.8 and 7.2 at an optimal temperature of 40-45°C. In a single batch biotransformation with suspended resting cells it was possible to produce 150g/L 3HPA as carbohydrazone at an overall productivity of 10.7gL(-1)hours(-1). In a single fed-batch biotransformation at 45°C 138g/L glycerol was converted into 108g/L 3HPA with an overall productivity of 21.6gL(-1)hours(-1). This is the highest 3HPA concentration and productivities reported so far for the microbial production of 3HPA from glycerol.

中文翻译:

罗伊氏乳杆菌从甘油中生产大量 3-羟基丙醛,大大提高了生物催化剂的寿命和生产率

3-羟基丙醛 (3HPA) 是一种有前途的多功能物质,来源于可再生原料甘油。它是罗伊氏乳杆菌中甘油代谢的产物。由于毒性作用,生物技术生产较差。在这项工作中,生物催化剂的寿命和产品形成可以大大增加。在已经应用的已建立的两步法中,细胞在第一步在厌氧条件下生长,在第二步中,固定或悬浮的生物催化剂在严格的厌氧条件下用于 3HPA 生产。在第一步中,可以达到 5.5g CDW/L (OD(600)≈23.4) 的生物质浓度。在第二步中,由于 3HPA 与细胞成分的相互作用,通常 3HPA 积累到有毒浓度并且反应在不到 60 分钟内停止。为了防止这种情况,有毒产品与新发现的清除剂碳酰肼结合形成腙。第一次可以在重复分批生物转化中将固定化生物催化剂回收至少十个循环(总寿命> 33 小时),总产量为 67 克 3HPA。在 40-45°C 的最佳温度下,最佳 pH 值介于 6.8 和 7.2 之间。在使用悬浮静息细胞的单批次生物转化中,可以以 10.7gL(-1) 小时 (-1) 的总生产率生产 150g/L 3HPA 作为碳腙。在 45°C 的单次补料分批生物转化中,138g/L 甘油转化为 108g/L 3HPA,总生产率为 21.6gL(-1) 小时 (-1)。这是迄今为止由甘油微生物生产 3HPA 所报道的最高 3HPA 浓度和生产率。有毒产物与新发现的清除剂碳酰肼结合形成腙。第一次可以在重复分批生物转化中循环固定化生物催化剂至少 10 个循环(总寿命 > 33 小时),总产量为 67 克 3HPA。在 40-45°C 的最佳温度下,最佳 pH 值介于 6.8 和 7.2 之间。在使用悬浮静息细胞的单批次生物转化中,可以以 10.7gL(-1) 小时 (-1) 的总生产率生产 150g/L 3HPA 作为碳腙。在 45°C 的单次补料分批生物转化中,138g/L 甘油转化为 108g/L 3HPA,总生产率为 21.6gL(-1) 小时 (-1)。这是迄今为止由甘油微生物生产 3HPA 所报道的最高 3HPA 浓度和生产率。有毒产物与新发现的清除剂碳酰肼结合形成腙。第一次可以在重复分批生物转化中循环固定化生物催化剂至少 10 个循环(总寿命 > 33 小时),总产量为 67 克 3HPA。在 40-45°C 的最佳温度下,最佳 pH 值介于 6.8 和 7.2 之间。在使用悬浮静息细胞的单批次生物转化中,可以以 10.7gL(-1) 小时 (-1) 的总生产率生产 150g/L 3HPA 作为碳腙。在 45°C 的单次补料分批生物转化中,138g/L 甘油转化为 108g/L 3HPA,总生产率为 21.6gL(-1) 小时 (-1)。这是迄今为止由甘油微生物生产 3HPA 所报道的最高 3HPA 浓度和生产率。
更新日期:2012-01-01
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