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Amphipols from A to Z.
Annual Review of Biophysics ( IF 10.4 ) Pub Date : 2011-05-07 , DOI: 10.1146/annurev-biophys-042910-155219
J-L Popot 1 , T Althoff , D Bagnard , J-L Banères , P Bazzacco , E Billon-Denis , L J Catoire , P Champeil , D Charvolin , M J Cocco , G Crémel , T Dahmane , L M de la Maza , C Ebel , F Gabel , F Giusti , Y Gohon , E Goormaghtigh , E Guittet , J H Kleinschmidt , W Kühlbrandt , C Le Bon , K L Martinez , M Picard , B Pucci , J N Sachs , C Tribet , C van Heijenoort , F Wien , F Zito , M Zoonens
Affiliation  

Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.

中文翻译:

两栖动物从A到Z。

Amphipols(APols)是短的两亲性聚合物,可替代清洁剂以使整体膜蛋白(MPs)保持水溶性。在这篇评论中,我们讨论了它们的结构和解决方案行为;他们与国会议员的联系方式;以及所得配合物的结构,动力学和溶液性质。迄今测试的所有MP均与APols形成水溶性复合物,与洗涤剂溶液中的MP相比,它们的生化稳定性通常得到大大改善。审查了功能和配体结合的APol捕获的MPs,并讨论了APols稳定MPs的机制。APol的应用包括MP折叠和无细胞合成,通过NMR进行结构研究,电子显微镜和X射线衍射,将MPs固定在固体支持物上的APol介导,蛋白质组学,
更新日期:2011-05-05
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