The aim of the study was to determine changes incurred by polyphenolic compounds from selected fruits in the lipid phase of the erythrocyte membrane, in liposomes formed of erythrocyte lipids and phosphatidylcholine liposomes. In particular, the effect of extracts from apple, chokeberry, and strawberry on the red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC liposomes was studied. In the erythrocyte population, the proportions of echinocytes increased due to incorporation of polyphenolic compounds. Fluorimetry with a laurdan probe indicated increased packing density in the hydrophilic phase of the membrane in presence of polyphenolic extracts, the highest effect being observed for the apple extract. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The polyphenolic extracts slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The studies have shown that the phenolic compounds contained in the extracts incorporate into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The compounds also penetrate the outer part of the external lipid layer of liposomes formed of natural and DPPC lipids, changing its packing order.

中文翻译：

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水果提取物在生物膜和模型膜的脂质相中引起的变化。

该研究的目的是确定来自选定水果的多酚化合物在红细胞膜的脂质相、红细胞脂质和磷脂酰胆碱脂质体形成的脂质体中引起的变化。特别是，苹果、苦莓和草莓提取物对红细胞形态、脂质亲水相的堆积顺序、疏水相的流动性以及 DPPC 脂质体中相变温度的影响是学习了。在红细胞群中，由于多酚化合物的掺入，棘红细胞的比例增加。使用 laurdan 探针的荧光测定表明，在多酚提取物存在的情况下，膜亲水相中的堆积密度增加，对苹果提取物观察到的效果最高。使用荧光探针 DPH 和 TMA-DPH，在膜的疏水相内没有注意到影响，因为脂质双层流动性没有改变。多酚提取物略微降低了磷脂酰胆碱脂质体的相变温度。研究表明，提取物中含有的酚类化合物进入红细胞膜的外部区域，影响其形状和脂质堆积顺序，这反映在棘红细胞数量的增加上。这些化合物还渗透到由天然和 DPPC 脂质形成的脂质体的外部脂质层的外部，改变其包装顺序。多酚提取物略微降低了磷脂酰胆碱脂质体的相变温度。研究表明，提取物中含有的酚类化合物进入红细胞膜的外部区域，影响其形状和脂质堆积顺序，这反映在棘红细胞数量的增加上。这些化合物还渗透到由天然和 DPPC 脂质形成的脂质体的外部脂质层的外部，改变其包装顺序。多酚提取物略微降低了磷脂酰胆碱脂质体的相变温度。研究表明，提取物中含有的酚类化合物进入红细胞膜的外部区域，影响其形状和脂质堆积顺序，这反映在棘红细胞数量增加上。这些化合物还渗透到由天然和 DPPC 脂质形成的脂质体的外部脂质层的外部，改变其包装顺序。