In vitro, carp blood was anticoagulated by using MgSO(4) at a final concentration of 22.2 mmol L(-1) and sodium citrate at a final concentration of 11.8 mmol L(-1) . The coagulation times for carp plasma diluted by ion-free water (1:1), and that of carp plasma to which thrombocytes and small lymphocytes were added, were measured at 23 °C using standard methods, and then contrasted with the coagulation times for plasma obtained from chickens and rabbits. The shapes of the thrombocytes and small lymphocytes, which were either wet mounted or stained with hematoxylin and eosin, were observed under a light microscope. We found that: (i) the coagulation reaction of carp blood was significantly (P < 0.01) accelerated by the addition of ion-free water; (ii) the three types of blood cells (thrombocytes, small lymphocytes and red blood cells) promoted plasma coagulation to a similar extent (P > 0.05); (iii) in carp Mg(2+) plasma and K(2) C(2) O(4) plasma, the thrombocytes were usually morphologically normal, but many small lymphocytes were destroyed and became aggregated; (iv) in the citrate plasma, thrombocytes were often aggregated, but the small lymphocytes were usually morphologically normal; and (v) the coagulation time for chicken and rabbit plasma was significantly extended by adding ion-free water.

中文翻译：

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鲤鱼（Cyprinus carpio）的凝血机制。

在体外，通过使用终浓度为22.2 mmol L（-1）的MgSO（4）和终浓度为11.8 mmol L（-1）的柠檬酸钠对鲤鱼血液进行抗凝。用标准方法在23°C下测量用无离子水（1：1）稀释的鲤鱼血浆的凝结时间以及添加了血小板和小淋巴细胞的鲤鱼血浆的凝结时间，然后与从鸡和兔子身上获得的血浆。在光学显微镜下观察湿安装或苏木精和曙红染色的血小板和小淋巴细胞的形状。我们发现：（i）添加无离子水可显着促进鲤鱼血液的凝结反应（P <0.01）；（ii）三种类型的血细胞（血小板，小淋巴细胞和红细胞）促进血浆凝结的程度相似（P> 0.05）；（iii）在鲤鱼Mg（2+）血浆和K（2）C（2）O（4）血浆中，血小板通常形态正常，但许多小淋巴细胞被破坏并聚集；（iv）在柠檬酸盐血浆中，血小板通常聚集，但小淋巴细胞通常形态正常；（v）添加无离子水可显着延长鸡和兔子血浆的凝血时间。但是小淋巴细胞通常在形态上是正常的。（v）添加无离子水可显着延长鸡和兔子血浆的凝血时间。但是小淋巴细胞通常在形态上是正常的。（v）添加无离子水可显着延长鸡和兔子血浆的凝血时间。