Here, we report a new method of concentration-enhanced binding kinetics for a rapid immunoassay screening test on a gold surface in a poly(dimethylsiloxane) (PDMS) microfluidic chip format. The use of alkylthiolate self-assembled monolayers on gold surfaces of a PDMS-glass microchip resulted in accelerated binding kinetics of Human chorionic gonadotropin (hCG) at an electrokinetic trapping zone. We used a PBS solution (buffer concentration ~ 150 mM), not a dibasic buffer system (~10 mM), for the dynamic preconcentrating operation and the preconcentration of cy3 labeled streptavidin onto biotinylated Au surface revealed that the binding kinetics of the protein were linearly proportional to the concentration profile of the preconcentration plug. We showed rapid detection of hCG in the clinical range with a shorten assay time of 10 min. Also, we demonstrated that the amount of sample needed were detection was decreased from ~4 mL to ~25 μL in the standard serum tests. The enhanced binding kinetics between hcG Ag-Ab via preconcentration showed good feasibility for use in a rapid immunoassay screening test.

中文翻译：

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使用纳米流体预浓缩器对 Au 表面上的人绒毛膜促性腺激素 (hCG) 进行浓度增强的快速检测。

在这里，我们报告了一种新的浓度增强结合动力学方法，用于在聚二甲基硅氧烷 (PDMS) 微流控芯片格式的金表面上进行快速免疫测定筛选测试。在 PDMS 玻璃微芯片的金表面上使用烷基硫醇盐自组装单层导致人绒毛膜促性腺激素 (hCG) 在电动捕获区的结合动力学加速。我们使用 PBS 溶液（缓冲液浓度 ~ 150 mM），而不是二元缓冲系统（~10 mM），用于动态预浓缩操作和 cy3 标记的链霉亲和素在生物素化 Au 表面上的预浓缩表明蛋白质的结合动力学是线性的与预浓缩塞的浓度分布成正比。我们展示了在临床范围内快速检测 hCG，缩短了 10 分钟的检测时间。还，我们证明，在标准血清测试中，检测所需的样品量从~4 mL 减少到~25 μL。通过预浓缩增强 hcG Ag-Ab 之间的结合动力学显示出用于快速免疫测定筛选试验的良好可行性。