Primary alveolar type of rhabdomyosarcoma (RMS) tumor tissue was collected from the tongue of a 17-year-old Japanese woman and used to successfully establish a rhabdomyosarcoma cell line, which has been designated NUTOS. The chromosomal distribution revealed that the NUTOS cell line was hyper-tetraploid with chromosomal translocation. The cells were grown in Dulbecco’s modified eagle medium/F12 supplemented with 15% fetal bovine serum, 0.1% non-essential amino acids solution (NEAA), 50 μg of streptomycin, 50 U/mL of penicillin and 0.25 μg /mL of Fungizone. The NUTOS shapes included small spindles, large spindles and long, thick multinucleated cells. All three cell types were immunostained with anti-desmin antibody, which is a marker protein for middle sized myofilaments. Furthermore, immunocytochemical staining revealed that the cells were positively immunostained with anti-MyoD, myogenin, α-sarcomeric actin, myosin and troponin T. Mitotic figures were only observed in the small spindle cells. These cells were coadunated with each other at the lateral portion of the apex of the cells. Subsequently, these cells grew into large multinucleated cells. Autonomic contractions (approximately 20 times/min) were observed in both the large spindle cells and the large multinucleated cells. NUTOS cells incorporated serotonin from the serum in the growth medium. Histopathological observations of the NUTOS cell grafts in the subcutis of nude mice exhibited characteristics similar to those seen for the primary rhabdomyosarcoma of the tongue. Susceptibility tests for the anti-cancer drugs revealed that NUTOS cells were susceptive to cisplatin, paclitaxel, and docetaxel, but not to adriacin.

中文翻译：

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人舌肉瘤来源的横纹肌肉瘤细胞系 NUTOS 的建立和表征：特别参考抗癌药物的敏感性。

从一名 17 岁日本女性的舌头中收集原发性肺泡型横纹肌肉瘤 (RMS) 肿瘤组织，并用于成功建立横纹肌肉瘤细胞系，该细胞系已命名为 NUTOS。染色体分布显示 NUTOS 细胞系是具有染色体易位的超四倍体。细胞在补充有 15% 胎牛血清、0.1% 非必需氨基酸溶液 (NEAA)、50 μg 链霉素、50 U/mL 青霉素和 0.25 μg/mL Fungizone 的 Dulbecco 改良 eagle 培养基/F12 中生长。NUTOS 形状包括小纺锤体、大纺锤体和长而厚的多核细胞。所有三种细胞类型都用抗结蛋白抗体进行免疫染色，抗结蛋白抗体是中等大小肌丝的标记蛋白。此外，免疫细胞化学染色显示细胞被抗-MyoD、肌细胞生成素、α-肌节肌动蛋白、肌球蛋白和肌钙蛋白T免疫染色呈阳性。仅在小的梭形细胞中观察到有丝分裂。这些细胞在细胞顶端的横向部分彼此共生。随后，这些细胞长成大的多核细胞。在大梭形细胞和大多核细胞中均观察到自主神经收缩（约 20 次/分钟）。NUTOS 细胞将血清中的血清素掺入生长培养基中。裸鼠皮下组织中 NUTOS 细胞移植物的组织病理学观察表现出与舌部原发性横纹肌肉瘤相似的特征。