There are few case reports describing small cell lung carcinoma (SCLC), which secrete parathyroid hormone (PTH)-related protein (PTHrP) and result in hypercalcemia. We have established a novel cell line, derived from a 37-year-old woman with SCLC, which produced PTH, PTH-rP, and a part of proopiomelanocortin (POMC), and led to hypercalcemia. The cell line, named SS-1, was grown as floating cell clusters in DMEM/F12 medium supplemented with 10% fetal bovine serum and had a population doubling time of 72 h. The modal chromosome number was 47 (88%); marker chromosomes were not observed. The SS-1 cell line secreted not only PTHrP but also PTH, and both were decreased by CaCl₂ administration. Decreasing the concentration of Ca⁺⁺ in the growth medium stimulated the secretion of both PTHrP and PTH. The cell line had calcium sensing receptor (Cas-R). Since PTHrP and PTH secretion from the SS-1 cells was related to Ca⁺⁺ concentration in the growth medium, the cell line might be useful for the study of PTH-rP and PTH regulation as well as for SCLC analysis. In addition, the cells secreted N terminal POMC, the precursor of adrenocorticotropic hormone, in response to stimulation with corticotropin releasing hormone. In summary, we established a novel cell line, SS-1 from SCLC, which produced PTHrP, PTH and N terminal POMC.

中文翻译：

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源自人类小细胞肺癌的新型细胞系的建立和表征，该细胞系分泌甲状旁腺激素、甲状旁腺激素相关蛋白和阿片黑素原。

很少有病例报告描述小细胞肺癌 (SCLC)，它会分泌甲状旁腺激素 (PTH) 相关蛋白 (PTHrP) 并导致高钙血症。我们已经建立了一个新的细胞系，该细胞系源自一名 37 岁的小细胞肺癌女性，它产生 PTH、PTH-rP 和一部分阿片黑素皮质素原 (POMC)，并导致高钙血症。名为 SS-1 的细胞系在补充有 10% 胎牛血清的 DMEM/F12 培养基中作为漂浮细胞簇生长，群体倍增时间为 72 小时。模态染色体数为 47 (88%)；未观察到标记染色体。SS-1 细胞系不仅分泌 PTHrP，还分泌 PTH，两者都因 CaCl ₂给药而减少。降低 Ca ⁺⁺的浓度在生长培养基中刺激了 PTHrP 和 PTH 的分泌。该细胞系具有钙感应受体 (Cas-R)。由于来自 SS-1 细胞的 PTHrP 和 PTH 分泌与生长培养基中的Ca ⁺⁺浓度有关，因此该细胞系可能有助于研究 PTH-rP 和 PTH 调节以及用于 SCLC 分析。此外，细胞分泌 N 末端 POMC，促肾上腺皮质激素的前体，以响应促肾上腺皮质激素释放激素的刺激。总之，我们建立了一个新的细胞系，来自 SCLC 的 SS-1，它产生 PTHrP、PTH 和 N 端 POMC。