In recent years, removal of ova or ovaries before chemotherapy or radiation therapy has been investigated in young female cancer patients to avoid the adverse effects of treatment. Orthotopic autotransplantation of ovarian cortex has advantages such as easy collection of ova and the possibility of spontaneous pregnancy. Although children have been born after successful orthotopic autotransplantation into the residual ovaries, some patients cannot undergo this procedure such as those who need bilateral ovariectomy or pelvic radiation therapy, therefore it is still necessary to investigate suitable heterotopic autotransplantation sites. The present study was performed in primates (cynomolgus monkeys) with the objective of determining the optimum site for heterotopic autotransplantation of ovarian cortex to enhance the clinical application of this method. The retroperitoneal iliac fossa and omentum were selected as sites for heterotopic autotransplantation. Two cynomolgus monkeys were subjected to laparotomy under anesthesia. After resection of the bilateral adnexae, the ovaries were cut into 0.5 cm cubes that were transplanted. Blood levels of follicle-stimulating hormone, luteinizing hormone, estradiol, and progesterone were monitored, and monkeys with a regular estrus cycle underwent superovulation and egg collection. In both monkeys studied, recovery of a regular estrus cycle was confirmed after heterotopic autotransplantation of ovarian tissue. Mll phase ova were successfully collected from tissues transplanted into the retroperitoneal iliac fossa or omentum. Development to the early blastocyst stage was confirmed after microfertilization. We established an appropriate method of heterotopic autotransplantation using ovarian cortex into the retroperitoneal iliac fossa or omentum in primates.

中文翻译：

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食蟹猴卵巢皮质的异位自体移植。

近年来，已经在年轻女性癌症患者中研究了在化疗或放疗前切除卵子或卵巢以避免治疗的不良反应。卵巢皮质原位自体移植具有取卵容易、可自然受孕等优点。残卵巢原位自体移植成功后虽然已有患儿出生，但部分患者如需双侧卵巢切除或盆腔放疗等不能进行此手术，因此仍需寻找合适的异位自体移植位点。本研究在灵长类动物（食蟹猴）中进行，目的是确定卵巢皮质异位自体移植的最佳部位，以提高该方法的临床应用。选择腹膜后髂窝和大网膜作为异位自体移植的部位。两只食蟹猴在麻醉下进行剖腹手术。切除双侧附件后，将卵巢切成0.5厘米的立方体进行移植。监测促卵泡激素、促黄体生成素、雌二醇和孕酮的血液水平，对发情周期规律的猴子进行超排卵和收集卵子。在所研究的两只猴子中，卵巢组织异位自体移植后，正常发情周期的恢复得到证实。从移植到腹膜后髂窝或大网膜的组织中成功收集了 Mll 期卵子。微量受精后证实发育到早期囊胚阶段。我们建立了一种适当的异位自体移植方法，使用卵巢皮质进入灵长类动物的腹膜后髂窝或网膜。