Novel cell lines, designated NM78-AM and NM78-MM, have been established from a malignant melanoma of the cheek oral mucosa. NM78-AM cells were spherical, grew in suspension as clusters, and produced no melanin. In contrast, NM78-MM cells were adherent and produced melanin granules. Initially, NM78-AM cells were grown on fibroblast feeder cells or in growth media supplemented with 10% conditioned medium from fibroblasts, but eventually grew in standard growth media alone. NM78-AM cells had interdigitating microvilli and formed cell clusters. They had large nucleoli, desmosomes, lipid droplets, and well-developed Golgi apparatuses. In contrast, NM78-MM cells grew as adherent neuron-like cells. They had large prominent nucleoli, irregular nuclear membranes, a number of mitochondria, well-developed Golgi apparatuses, melanosomes at various stages of development in the cytoplasm, and the cells secreted melanin granules. Projections from these melanotic cells formed anastomoses with each other. NM78-MM cells stained immunofluorescently for internexin, neuron specific enolase, NF-200, and glial fibrillary acidic protein. These cells were severely aneuploid, approximating to triploidy, and had many marker chromosomes. We used a real-time monitoring system to evaluate oxygen concentrations in culture medium to investigate the susceptibility of both cell lines to various anti-cancer drugs. NM78-AM cells were slightly sensitive to actinomycin D, but not to cisplatin, irinotecan, the irinotecan metabolite SN-38, taxol, taxotere, bleomycin and methotrexate; NM78-MM cells were sensitive to cisplatin, and not to taxol, taxotere, carboplatin, and irinotecan. These new cell lines, NM78-AM and NM78-MM, will be very important for the development of new chemotherapeutics for oral malignant melanoma.

中文翻译：

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源自人类口腔恶性黑色素瘤的新型无色素和黑色素细胞系 NM78-AM 和 NM78-MM。

新的细胞系，命名为 NM78-AM 和 NM78-MM，已经从脸颊口腔黏膜的恶性黑色素瘤中建立起来。NM78-AM 细胞呈球形，悬浮生长为簇，不产生黑色素。相比之下，NM78-MM 细胞粘附并产生黑色素颗粒。最初，NM78-AM 细胞在成纤维细胞饲养细胞或补充有 10% 来自成纤维细胞的条件培养基的生长培养基中生长，但最终仅在标准生长培养基中生长。NM78-AM 细胞具有交叉微绒毛并形成细胞簇。它们有大的核仁、桥粒、脂滴和发达的高尔基体。相比之下，NM78-MM 细胞生长为贴壁神经元样细胞。它们有大而突出的核仁、不规则的核膜、许多线粒体、发达的高尔基体、黑色素体在细胞质中处于不同发育阶段，细胞分泌黑色素颗粒。这些黑色素细胞的投射相互形成了吻合。NM78-MM 细胞对 internexin、神经元特异性烯醇化酶、NF-200 和胶质纤维酸性蛋白进行免疫荧光染色。这些细胞是严重的非整倍体，接近三倍体，并且有许多标记染色体。我们使用实时监测系统来评估培养基中的氧浓度，以研究两种细胞系对各种抗癌药物的敏感性。NM78-AM 细胞对放线菌素 D 略敏感，但对顺铂、伊立替康、伊立替康代谢物 SN-38、紫杉醇、泰索帝、博来霉素和甲氨蝶呤不敏感；NM78-MM 细胞对顺铂敏感，对紫杉醇、泰素帝、卡铂和伊立替康不敏感。