We describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, the Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.

中文翻译：

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用于细胞机械转导的金尖弹性体柱。

我们描述了一种用于制造弹性体柱阵列的技术，其顶部表面经过选择性化学功能化处理，以促进细胞力转导实验中的细胞粘附。该技术涉及创建由圆孔阵列组成的刚性模具，在其中沉积一层薄薄​​的 Au，同时模具的顶面和孔的侧壁由 Cr 牺牲层保护。当在模具中形成弹性体时，Au 粘附在模制支柱的顶部。然后可以用诱导细胞粘附的蛋白质选择性地将其功能化，而表面的其余部分则用驱虫物质处理。另一个好处是柱子的顶部可以进行荧光标记，以提高力转换测量的准确性。