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The influence of metabolically engineered glucosinolates profiles in Arabidopsis thaliana on Plutella xylostella preference and performance.
Chemoecology ( IF 1.6 ) Pub Date : 2009-11-12 , DOI: 10.1007/s00049-009-0028-4
Bejai R Sarosh , Ute Wittstock , Barbara Ann Halkier , Barbara Ekbom

The oviposition preference and larval performance of the diamondback moth (DBM), Plutella xylostella, was studied using Arabidopsis thaliana plants with modified glucosinolate (GS) profiles containing novel GSs as a result of the introduction of individual CYP79 genes. The insect parameters were determined in a series of bioassays. The GS content of the plants as well as the number of trichomes were measured. Multivariate analysis was used to determine the possible relationships among insect and plant variables. The novel GSs in the tested lines did not appear to have any unequivocal effect on the DBM. Instead, the plant characteristics that affected larval performance and larval preference did not influence oviposition preference. Trichomes did not affect oviposition, but influenced larval parameters negatively. Although the tested A. thaliana lines had earlier been shown to influence disease resistance, in this study no clear results were found for P. xylostella.

中文翻译:

拟南芥中代谢工程化的硫代葡萄糖苷谱对小菜蛾偏好和性能的影响。

产卵偏好和小菜蛾(DBM),幼虫性能小菜蛾,使用进行了研究拟南芥植物与含有新颖硫苷的引入个体的结果改性芥子油苷(GS)型材CYP79基因。在一系列生物测定中确定昆虫参数。测量植物的GS含量以及毛状体的数量。多变量分析用于确定昆虫和植物变量之间可能的关系。测试品系中的新型 GS 似乎对 DBM 没有任何明确的影响。相反,影响幼虫表现和幼虫偏好的植物特征并不影响产卵偏好。毛状体不影响产卵,但对幼虫参数产生负面影响。尽管测试的拟南芥品系早先已被证明会影响抗病性,但在本研究中没有发现小菜蛾的明确结果。
更新日期:2009-11-12
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