Cells can secrete biotherapeutic molecules that can replace or restore host function. The transplantation of such cells is a promising therapeutic modality for the treatment of several diseases including type 1 diabetes mellitus. These cellular grafts are encapsulated in semipermeable and immunoisolative membranes to protect them from the host immune system, while allowing the transport of nutrients and small molecules that are required for cell survival and function. The authors report on SU-8-based biocompatible immunoisolative cuboid microcontainers for cell transplantation. Each microcontainer comprises a 300×300×250 or a 1100×1100×250 μm(3) SU-8 hollowed cuboid base that houses the cells and an optically transparent SU-8-based nanoporous lid that closes the device. The hollowed cuboid base was formed by conventional optical lithography to have 8 nl (200×200×200 μm(3)) encapsulation volume for cellular payload. The lid comprises a thick SU-8 slab with an array of cylindrical wells, whose bottom surface is sealed with a thin nanoporous SU-8 membrane. The nanoporous membrane was created from a 100 nm grating (width and spacing) initial silicon mold subjected to a repeated cycle of oxidation and wet etching to achieve a 20 nm wide and 200 nm pitch nano silicon grating. Nanoimprinting and oblique-angle metal deposition, followed by inductively coupled plasma etching were utilized to create 15 nm wide and 350-450 nm deep nanoslots in the thin SU-8 membrane. Isolated mouse islets were encapsulated in the hollowed cuboid base and the nanoporous lid was assembled on top. The penetration of large and small molecules into the microcontainer was observed with fluorescence.

中文翻译：

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基于 SU-8 的免疫隔离微容器，具有由纳米压印光刻定义的纳米槽。

细胞可以分泌可以替代或恢复宿主功能的生物治疗分子。这种细胞的移植是治疗包括 1 型糖尿病在内的多种疾病的一种有前途的治疗方式。这些细胞移植物被封装在半透性和免疫隔离膜中，以保护它们免受宿主免疫系统的侵害，同时允许运输细胞存活和功能所需的营养物质和小分子。作者报告了用于细胞移植的基于 SU-8 的生物相容性免疫隔离长方体微容器。每个微容器包括一个 300×300×250 或 1100×1100×250 μm(3) SU-8 中空长方体底座，用于容纳细胞和一个光学透明的基于 SU-8 的纳米孔盖，用于关闭设备。中空的长方体底座由传统的光刻技术形成，具有 8 nl (200×200×200 μm(3)) 的蜂窝有效载荷封装体积。盖子包括一个厚的 SU-8 板，上面有一系列圆柱形孔，其底面用薄的纳米多孔 SU-8 膜密封。纳米多孔膜由 100 nm 光栅（宽度和间距）初始硅模具制成，经过重复的氧化和湿蚀刻循环，以实现 20 nm 宽和 200 nm 间距的纳米硅光栅。纳米压印和斜角金属沉积，然后是电感耦合等离子体蚀刻，用于在薄 SU-8 膜中创建 15 nm 宽和 350-450 nm 深的纳米槽。分离的小鼠胰岛被封装在中空的长方体底座中，纳米孔盖组装在顶部。