Previously undescribed oligosaccharides in bovine cheese whey permeate were characterized by a combination of nanoelectrospray Fourier Transform Ion Cyclotron Resonance (nESI-FTICR) mass spectrometry and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometry. Oligosaccharide composition was elucidated by collision-induced dissociation within the ICR cell. In addition to sialyllactose (the most abundant oligosaccharide in bovine colostrum), we identified 14 other oligosaccharides, half of which have the same composition of human milk oligosaccharides. These oligosaccharides could potentially be used as additives in infant formula and products for the pharmaceutical industry. Because whey permeate is a by-product from the production of whey protein concentrate (WPC) and is readily available, it is an attractive source of oligosaccharides for potential application in human nutrition.

中文翻译：

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奶酪乳清超滤渗透物是牛奶低聚糖的来源


通过纳电喷雾傅里叶变换离子回旋共振 (nESI-FTICR) 质谱和基质辅助激光解吸/电离傅里叶变换离子回旋共振 (MALDI-FTICR) 质谱相结合，对牛奶酪乳清渗透物中以前未描述的低聚糖进行了表征。通过 ICR 细胞内碰撞诱导的解离阐明了寡糖组成。除了唾液酸乳糖（牛初乳中最丰富的低聚糖）之外，我们还鉴定了 14 种其他低聚糖，其中一半具有与人乳低聚糖相同的成分。这些低聚糖有可能用作婴儿配方奶粉和制药行业产品的添加剂。由于乳清渗透物是浓缩乳清蛋白 (WPC) 生产过程中的副产品，并且易于获取，因此它是一种极具吸引力的低聚糖来源，具有潜在的人类营养应用价值。