Lactobacilli belong to the lactic acid bacteria, which play a key role in industrial and artisan food raw-material fermentation, including a large variety of fermented dairy products. Next to their role in fermentation processes, specific strains of Lactobacillus are currently marketed as health-promoting cultures or probiotics. The last decade has witnessed the completion of a large number of Lactobacillus genome sequences, including the genome sequences of some of the probiotic species and strains. This development opens avenues to unravel the Lactobacillus-associated health-promoting activity at the molecular level. It is generally considered likely that an important part of the Lactobacillus effector molecules that participate in the proposed health-promoting interactions with the host (intestinal) system resides in the bacterial cell envelope. For this reason, it is important to accurately predict the Lactobacillus exoproteomes. Extensive annotation of these exoproteomes, combined with comparative analysis of species- or strain-specific exoproteomes, may identify candidate effector molecules, which may support specific effects on host physiology associated with particular Lactobacillus strains. Candidate health-promoting effector molecules of lactobacilli can then be validated via mutant approaches, which will allow for improved strain selection procedures, improved product quality control criteria and molecular science-based health claims.

中文翻译：

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乳杆菌的细胞外生物学。

乳酸杆菌属于乳酸菌，它在工业和手工业食品原料发酵中起着关键作用，包括多种发酵乳制品。除了其在发酵过程中的作用外，乳杆菌的特定菌株目前还作为促进健康的培养物或益生菌进行销售。过去十年见证了许多乳酸杆菌基因组序列的完成，包括某些益生菌物种和菌株的基因组序列。这一发展为在分子水平上揭示与乳杆菌相关的健康促进活性开辟了途径。通常认为，与提议的与宿主（肠道）系统促进健康互动的乳杆菌效应分子的重要部分可能位于细菌细胞膜中。因此，准确预测乳杆菌外蛋白质组很重要。这些外蛋白质组的广泛注释，加上对物种或菌株特异性外蛋白质组的比较分析，可以确定候选效应分子，这可能支持对与特定乳杆菌菌株有关的宿主生理学的特定作用。然后可以通过突变方法验证乳杆菌的促进健康的效应分子，这将有助于改进菌株选择程序，改进产品质量控制标准和基于分子科学的健康主张。可以鉴定候选效应分子，其可以支持对与特定乳杆菌菌株有关的宿主生理的特定作用。然后可以通过突变方法验证乳杆菌的促进健康的效应分子，这将有助于改进菌株选择程序，改进产品质量控制标准和基于分子科学的健康主张。可以鉴定候选效应分子，其可以支持对与特定乳杆菌菌株有关的宿主生理的特定作用。然后可以通过突变方法验证乳杆菌的促进健康的效应分子，这将有助于改进菌株选择程序，改进产品质量控制标准和基于分子科学的健康主张。