Much recent evidence suggests that bone cells sense their mechanical environment via interstitial fluid flow. In this review, we summarize theoretical and experimental approaches to quantify fluid and solute transport in bone, starting with the early investigations of fluid shear stress applied to bone cells. The pathways of bone interstitial fluid and solute movement are high-lighted based on recent theoretical models, as well as a new generation of tracer experiments that have clarified and refined the structure and function of the osteocyte pericellular matrix. Then we trace how the fluid-flow models for mechanotransduction have evolved as new ultrastructural features of the osteocyte lacunar-canalicular porosity have been identified and how more recent in vitro fluid-flow and cell-stretch experiments have helped elucidate at the molecular level the possible pathways for cellular excitation in bone.

中文翻译：

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骨中的液体和溶质运输：流动诱导的机械传导


最近的许多证据表明，骨细胞通过间质液流动来感知其机械环境。在这篇综述中，我们从应用于骨细胞的流体剪切应力的早期研究开始，总结了量化骨中液体和溶质转运的理论和实验方法。基于最新的理论模型以及阐明和细化骨细胞周基质的结构和功能的新一代示踪剂实验，突出显示了骨间质液和溶质运动的途径。然后，我们追踪了随着骨细胞腔隙-小管孔隙度的新超微结构特征的确定，机械转导的流体流动模型是如何演变的，以及最近的体外流体流动和细胞拉伸实验如何帮助在分子水平上阐明了可能的结果。骨中细胞兴奋的途径。