The last several years have seen a significant increase in the number of reports describing the use of cationic polymers to design new materials and nanoscale assemblies that promote the surface-mediated delivery of DNA to cells and tissues. In general, these approaches fall into one of two broad categories: (i) methods based upon the physical adsorption of preformed, colloidal aggregates of cationic polymer and DNA (polyplexes) to surfaces, and (ii) methods for the layer-by-layer adsorption of DNA and cationic polymers on surfaces to fabricate multilayered thin films that provide control over the release of DNA into solution or to cells. In this Opinion, we discuss several recent examples of each of these approaches and provide commentary on ways in which the physical and chemical behaviours of cationic polymers have played key roles and present future opportunities to develop new methods for localized and surface-mediated cell transfection in vitro and in vivo.

中文翻译：

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DNA 的表面介导传递：阳离子聚合物负责。


过去几年，描述使用阳离子聚合物来设计新材料和纳米级组件以促进表面介导的 DNA 递送至细胞和组织的报告数量显着增加。一般来说，这些方法属于两大类之一：(i) 基于阳离子聚合物和 DNA（聚合复合物）的预制胶体聚集体物理吸附到表面的方法，以及 (ii) 逐层吸附的方法将 DNA 和阳离子聚合物吸附在表面上以制造多层薄膜，从而控制 DNA 释放到溶液或细胞中。在本意见中，我们讨论了每种方法的几个最近的例子，并对阳离子聚合物的物理和化学行为发挥关键作用的方式进行了评论，并提出了未来开发局部和表面介导细胞转染新方法的机会。体外和体内。