Miscanthus sinensis is a promising species for biomass production. Influences of genetic and nongenetic factors on androgenesis induction efficiency were investigated. This is the first report on successful induction of pollen-derived callus inM. sinensis. The callus yield was strongly affected by genotype. A beneficial influence of cold pretreatment of spikes on androgenesis induction was observed. The highest yield of calli was obtained in cultures on a modified C17 medium. The results suggest that the high callus yield might be caused by the late culture initiation. The beginning of anther culture at the end of the flowering season caused a 17-fold increase in callus yield, in comparison to culture initiated at the height of the flowering season (August). It is likely, however, that the efficiency of androgenesis induction in the case ofM. sinensis anther culture beginning in October could be related to a positive influence of growing donor plants in conditions of cooler and shorter day, i.e. 11-h day with temperature around 11°C and 13-h night with temperature around 5°C. Results of this study can significantly support the development of effective methods ofM. sinensis haploidization, which could be used in crop improvement by breeding.

中文翻译：

---

影响芒草（Anderss.）花药培养物中愈伤组织诱导的遗传和非遗传因素。

芒草是一种很有前途的生物质生产树种。研究了遗传和非遗传因素对雄激素诱导效率的影响。首次成功诱导中华花粉愈伤组织的报道. 愈伤组织产量受基因型的强烈影响。观察到穗冷预处理对雄激素诱导的有益影响。在改良的 C17 培养基上的培养物中获得了最高的愈伤组织产量。结果表明，高愈伤组织产量可能是由于培养开始晚造成的。与开花季节高峰期（8 月）开始的培养相比，在开花季节结束时开始花药培养使愈伤组织产量增加了 17 倍。然而，在M. sinensis的情况下，雄激素诱导的效率很可能10 月开始的花药培养可能与在凉爽和较短白天条件下种植供体植物的积极影响有关，即 11 小时白天温度在 11°C 左右，夜间 13 小时温度在 5°C 左右。本研究的结果可以显着支持开发有效的中华白菜单倍体化方法，该方法可用于通过育种进行作物改良。