An on-probe pyrolyzer has been constructed and interfaced with Desorption Electrospray Ionization (DESI) Mass Spectrometry (MS) for the rapid analysis of non-volatile pyrolysis products. The detection and analysis of non-volatile pyrolysis products of peptides, proteins and the synthetic polymer poly(ethylene glycol) are demonstrated with this instrument. The on-probe pyrolyzer can be operated off-line or on-line with the DESI source and was interfaced with a tandem MS (MS/MS) instrument, which allowed for structure characterization of the non-volatile pyrolytic products. Advantages of this system are its simplicity and speed of analysis since the pyrolysis is performed in situ on the DESI source probe and hence, it avoids extraction steps and/or the use of matrices (e.g., as in MALDI-MS analyses).

中文翻译：

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用于分析非挥发性热解产物的探针上热解解吸电喷雾电离 (DESI) 质谱

已构建探针上热解器并与解吸电喷雾电离 (DESI) 质谱 (MS) 连接，以快速分析非挥发性热解产物。该仪器演示了肽、蛋白质和合成聚合物聚乙二醇的非挥发性热解产物的检测和分析。探针上的热解器可以与 DESI 源脱机或联机操作，并与串联 MS (MS/MS) 仪器连接，允许对非挥发性热解产物进行结构表征。该系统的优点是其简单和分析速度快，因为热解是在 DESI 源探针上原位进行的，因此它避免了提取步骤和/或基质的使用（例如，在 MALDI-MS 分析中）。