The vast majority of clinical tissue samples are formalin-fixed and paraffin-preserved. This type of preservation has been considered an obstacle to protein extraction from these tissues. However, these are the very tissue samples that have associated patient histories, diagnoses and outcomes - ideal samples in the quest to translate bench research into clinical applications. Thus, until recently, these valuable specimens have been unavailable for proteomic analysis.Over the last decade, researchers have been exploring efficient methods to undo protein cross-linking caused by standard tissue fixatives and extract proteins from archived tissue specimens. These methods have been applied in different clinical proteomic studies. In this report, we attempt to review the development of these techniques, summarize the proteomic findings, and discuss the impact on future clinical proteomics.

中文翻译：

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固定组织标本的蛋白质组学——综述

绝大多数临床组织样本是福尔马林固定和石蜡保存的。这种类型的保存被认为是从这些组织中提取蛋白质的障碍。然而，这些正是与患者病史、诊断和结果相关联的组织样本 - 寻求将实验室研究转化为临床应用的理想样本。因此，直到最近，这些有价值的标本一直无法用于蛋白质组学分析。在过去十年中，研究人员一直在探索消除由标准组织固定剂引起的蛋白质交联并从存档的组织标本中提取蛋白质的有效方法。这些方法已应用于不同的临床蛋白质组学研究。在本报告中，我们试图回顾这些技术的发展，总结蛋白质组学的发现，