The optical setup and the performance of a prototype UV/Vis multiwavelength analytical ultracentrifuge (MWL-AUC) is described and compared to the commercially available Optima XL-A from Beckman Coulter. Slight modifications have been made to the optical path of the MWL-AUC. With respect to wavelength accuracy and radial resolution, the new MWL-AUC is found to be comparable to the existing XL-A. Absorbance accuracy is dependent on the light intensity available at the detection wavelength as well as the intrinsic noise of the data. Measurements from single flashes of light are more noisy for the MWL-AUC, potentially due to the absence of flash-to-flash normalization in the current design. However, the possibility of both wavelength and scan averaging can compensate for this and still give much faster scan rates than the XL-A. Some further improvements of the existing design are suggested based on these findings.

中文翻译：

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用于分析超速离心机的基于快速光纤的紫外/可见多波长检测器的性能

描述了原型 UV/Vis 多波长分析超速离心机 (MWL-AUC) 的光学设置和性能，并与 Beckman Coulter 的市售 Optima XL-A 进行了比较。MWL-AUC 的光路略有修改。在波长精度和径向分辨率方面，发现新的 MWL-AUC 与现有的 XL-A 相当。吸光度精度取决于检测波长下可用的光强度以及数据的固有噪声。对于 MWL-AUC，单次闪光的测量噪声更大，这可能是由于当前设计中没有闪光到闪光归一化。然而，波长和扫描平均的可能性可以弥补这一点，并且仍然提供比 XL-A 快得多的扫描速率。